Glutathione gsh assay kit

Ferrostain‐1 (Fer‐1, #S7243, an inhibitor of ferroptosis), liproxstatin‐1 (Lip‐1, #S7699, an inhibitor of ferroptosis), necrostatin‐1 (Nec‐1, #S8087, an inhibitor of necroptosis), Z‐VAD‐FMK (Z‐VAD, #S7023, an inhibitor of apoptosis), chloroquine (CQ, #S6999; an inhibitor of autophagy) and 2′, 7’‐Dichlorodihydrofluorescein diacetate (DCFH‐DA, #S9687) were purchased from Selleck (Houston, TX, USA). Cell Counting Kit 8 (CCK8) (#ab228554), Lactate Dehydrogenase (LDH) Assay Kit (#ab102526), Lipid Peroxidation Assay Kit (#ab118970), 12(S)‐hydroxyeicosatetraenoic acid (HETE) ELISA Kit (#ab133034), 15(S)‐HETE ELISA Kit (#ab133035), Iron Assay Kit (#ab83366) and Glutathione (GSH) Assay Kit (#ab239727) were purchased from Abcam (Cambridge, UK). CytoSelect™ 24‐Well Cell Migration and Invasion Assay Combo Kit (#CBA‐100) was purchased from Cell Biolabs (San Diego, CA, USA).

LPS (Escherichia coli O111: B4, #L2630) was purchased from Sigma-Aldrich (St Louis, MO, USA). Recombinant Mouse GDF7 Protein (rmGDF7, #779-G7), Mouse IL-6 Quantikine ELISA Kit (#M6000B), and Mouse TNF-α Quantikine ELISA Kit (#MTA00B) were purchased from R&D Systems, Inc. (Minneapolis, MN, Canada). Compound C (CpC, #HY-13418A) was purchased from MedChemExpress (Monmouth Junction, NJ, USA), and the structure was provided on the official website: https://www.medchemexpress.cn/dorsomorphin.html. Mouse GDF7 ELISA Kit (#MBS2500588) was purchased from MyBioSource, Inc. (San Diego, CA, USA). Lactate Dehydrogenase (LDH) Assay Kit (#ab102526), Mouse Myeloperoxidase (MPO) ELISA Kit (#ab155458), Lipid Peroxidation (MDA) Assay Kit (#ab118970), Lipid Peroxidation (4-HNE) Assay Kit (#ab238538), Superoxide Dismutase (SOD) Activity Assay Kit (#ab65354), and Glutathione (GSH) Assay Kit (#ab239727) were purchased from Abcam (Cambridge, UK). ROS Assay Kit (#S0033) was purchased from Beyotime (Shanghai, China). Pierce™ BCA Protein Assay Kit (#23225) was purchased from Thermo Fisher Scientific (San Jose, CA, USA).