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Lumplfln 60 w

Manufactured by Olympus
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Sourced in Japan

The LUMPLFLN 60×W is a water immersion objective lens designed for Olympus microscopes. It has a high numerical aperture of 1.0 and a working distance of 2.0 mm, which allows for the high-resolution imaging of specimens in an aqueous environment.

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Lab products found in correlation

Ba510 550 bandpass emission filter, by Olympus (1 mentions) Ixonem, by Oxford Instruments (1 mentions) Bx51wi, by Olympus (1 mentions) Nipkow confocal scanner csu21, by Yokogawa (1 mentions) Mvs 700, by PerkinElmer (1 mentions)

2 protocols using lumplfln 60 w

1

In Vivo Nanoimaging of Cardiomyocytes

Cited 1 time
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Our microscopic system for in vivo nanoimaging was described in detail in our previous studies (Kobirumaki-Shimozawa et al., 2016 (link); Shimozawa et al., 2017 (link)). In brief, an upright microscope (BX-51WI; Olympus Co.) combined with a Nipkow confocal scanner (CSU21; Yokogawa Electric Co.) and an electron-multiplying charge-coupled device camera (iXonEM+; Andor Technology Ltd.) were used at a 512 × 170–pixel resolution at an exposure time of 9.8 ms. A 60× water immersion lens (LUMPLFLN 60×W, numerical aperture 1.00; Olympus Co.) was used to visualize the LV surface. α-Actinin-AcGFP–expressing myocytes in the left ventricle were excited by a 488-nm laser light (HPU50211-PFS; Furukawa Electric Co.), and the resultant fluorescence signals were detected by using a BA510–550 bandpass emission filter (Olympus Co.).
Kobirumaki-Shimozawa F., Shimozawa T., Oyama K., Baba S., Li J., Nakanishi T., Terui T., Louch W.E., Ishiwata S, & Fukuda N. (2021). Synchrony of sarcomeric movement regulates left ventricular pump function in the in vivo beating mouse heart. The Journal of General Physiology, 153(11), e202012860.
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2

High-Speed Imaging of Oscillating Polymer Microbubbles

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A high-speed imaging system (UPMC Cam), described previously (24 (link)), was used to acquire movies of the oscillating polymer MBs during US excitation. The camera system is capable of imaging microscopic movies at 25 million frames per second (Mfps) for 128 frames and a frame size of 920×616 pixels. A strobe light (MVS-700, PerkinElmer, Salem, MA) triggered from the camera system timing board served as the light source. A modular upright microscope (BXFM, Olympus Corp, Tokyo, Japan) equipped with water immersion lenses (LUMPLFLN 10×/W, LUMPLFLN 60×/W, Olympus) was used to image the center of the cellulose tubing holding the samples.
Chen X., Chen X., Wang J., Yu F.T., Villanueva F.S, & Pacella J.J. (2023). The dynamic behavior of polymer microbubbles during long ultrasound tone-burst excitation and its application for sonoreperfusion therapy. Ultrasound in medicine & biology, 49(4), 996-1006.
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