Phosphate buffer saline 1x

Phosphate buffer saline (PBS) 1X (pH 7.4), bovine serum albumin (BSA), and TRIS Buffer (50 mM Tris–HCl, 2 mM MgCl₂, 150 mM NaCl pH 7.5) were purchased from Sigma-Aldrich (Milan, Italy).
The aptamer AP_7462DNA, 5′-GCGGCGCGGTATGGAATTAGTGACCTTCCGCGCGCCCCATTTTTTATAGGGGCCGC-3′, was synthetized by Metabiom international AG (Planegg, Germany) and used for the electrochemical tests.
The aptamer with the addition at 5′ end of C6 S–S-polyT and a 6-Carboxytetramethylrhodamine (TAMRA) at 3′ end (named aptamer-TAMRA) was purchased from GenScript Biotech (Leiden GenScript Biotech, Leiden) and used for the contact angle and fluorescence experiments.
A solution of potassium ferrocyanide (K₄[Fe(CN)₆ × 3H₂O]) 10 mM prepared using sterilized PBS buffer 1X (AnalytiCals, Milan, Italy) was used as redox probe in the electrochemical tests.
The electrochemical analysis was conducted using an Potentiostat/Galvanostat/Impedance Analyzer (Metrohm Italia, VA, Italy) and screen-printed carbon electrodes (110, Metrohm Italia, VA, Italy).

Phosphate buffer saline (PBS) 1X (pH 7.4) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (Milan, Italy). DNA primers and probe were synthesized by MWG-Biotech (Ebersberg, Germany). Reagents for PCR were purchased from Applied Biosystems (Milan, Italy). The nylon membrane (Zeta-Probe GT; Bio-Rad, Milan, Italy) and Dig Easy Hyb buffer (Roche Diagnostics, Monza, Italy) were used in the dot blot tests. For the biosensor construction, PEDOT:PSS (Clevios™ PH500, Starck GmbH, Cologne, Germany), ethylene glycol (Sigma-Aldrich, Milan, Italy), dodecyl benzene sulfonic acid (DBSA) (Sigma-Aldrich, Milan, Italy), and PBS 1X were used. All media used for the microbiological analysis were purchased from Oxoid (Milan, Italy). BioNano gold-coated borosilicate coverslips of 15 mm × 13 mm with gold thickness of 50 nm on a glass substrate of 15 mm × 15 mm × 0.15 mm (PHASIS, Geneva, Switzerland) were used to build the textile OECT biosensor.