Phosphate citrate buffer tablet p4809 50tab

Gold (III) chloride trihydrate (520918–5G), trisodium citrate dihydrate (S1804–500G), O-phenylenediamine (OPD) powder (P9029–50G), tween 20 (P1379–1L) and phosphate-citrate buffer tablet (P4809–50TAB) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Consensus M2e peptide (acetylated-SLLTEVETPIRNEWGSRSNDSSDC-amidated, MW: 2736 Da) was chemically synthesized at 95% purity by AAPPTec (Louisville, KY, USA). Mouse CpG oligodeoxynucleotide (ODN) 1826 (5’-TCCATGACGTTCCTGACGTT-3’) and the ferret CpG ODN 2006 (5’-TCGTCGTTTTGTCGTTTTGTCGTT-3’), recognized by toll-like receptor 9 (TLR9), were synthesized by Integrated DNA Technologies (Coralville, IA, USA). Horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibodies were bought from Southern Biotech (Birmingham, AL, USA). HRP-conjugated goat anti-ferret IgG was purchased from KPL Inc. (Gaithersburg, MD, USA). ELISA plate blocking buffer concentrate (80061) was obtained from Alpha Diagnostic International (San Antonio, TX, USA). 10X Tris/Boric acid/EDTA (1610733) was purchased from BioRad. 10X Phosphate buffered saline (PBS) (46013CM) was purchased from Fisher Scientific. Milli-Q water with a resistance of 18.2 MΩ cm was used in all the experiments.

Gold(III) chloride trihydrate (520918-5G), trisodium citrate dihydrate (S1804-500G) and phosphate-citrate buffer tablet (P4809-50TAB) were purchased from Sigma-Aldrich. Sodium chloride (BDH0286-500G), tween 20 (BP337-100) and dithiothreitol (20289) were purchased from Fisher Scientific. O-Phenylenediamine (OPD) (00-2003) was purchased from Invitrogen. Milli-Q water with a resistance of 18.2 MΩ cm was used in all the experiments.