Procartaplex human tgf beta 1 simplex kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The ProcartaPlex Human TGF-beta 1 Simplex Kit is a multiplex assay designed to measure the concentration of TGF-beta 1 in human samples. It utilizes bead-based technology to enable the quantitative detection of the target analyte.

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Lab products found in correlation

Procartaplex human cytokine panel 1b, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

TGF]-ß1 (76 citations) ProcartaPlex kit (18 citations) ProcartaPlex™ Simplex Kit (10 citations) TGF-beta 1 (6 citations)

2 protocols using procartaplex human tgf beta 1 simplex kit

Multiplex Cytokine Quantification in Blood Plasma

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Blood plasma was diluted 1:3, and IL-21, IFN-α, and IFN-y were detected using a bead-based multiplex cytokine array (ProcartaPlex Human Cytokine Panel 1B, Thermo Fisher Scientific). TGF-β was detected using the ProcartaPlex Human TGF-beta 1 Simplex Kit (Thermo Fisher Scientific). Prior to measuring plasma-TGF-β1, the bioactive form of TGF-β1 was generated by incubating the plasma with 1 N HCl followed by neutralization with 1.2 N NaOH according to the manufacturer’s instructions. All cytokines were measured using the Luminex MAGPIX instrument and quantified using the xPONENT analysis software (Luminex Corporation).

Ferreira-Gomes M., Kruglov A., Durek P., Heinrich F., Tizian C., Heinz G.A., Pascual-Reguant A., Du W., Mothes R., Fan C., Frischbutter S., Habenicht K., Budzinski L., Ninnemann J., Jani P.K., Guerra G.M., Lehmann K., Matz M., Ostendorf L., Heiberger L., Chang H.D., Bauherr S., Maurer M., Schönrich G., Raftery M., Kallinich T., Mall M.A., Angermair S., Treskatsch S., Dörner T., Corman V.M., Diefenbach A., Volk H.D., Elezkurtaj S., Winkler T.H., Dong J., Hauser A.E., Radbruch H., Witkowski M., Melchers F., Radbruch A, & Mashreghi M.F. (2021). SARS-CoV-2 in severe COVID-19 induces a TGF-β-dominated chronic immune response that does not target itself. Nature Communications, 12, 1961.

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Quantitative Multiplex Assay for Exosomal Growth Factors

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Growth factors such as fibroblast growth factor 2 (FGF-2), hepatocyte growth factor (HGF), platelet-derived growth factor-BB (PDGF-BB), vascular endothelial growth factor A (VEGF-A), and transforming growth factor beta (TGF-β) were measured by Luminex assay using ProcartaPlex^TM Multiplex Immunoassays (Human Custom ProcartaPlex 4—Plex Kit and ProcartaPlex Human TGF beta 1 Simplex Kit—Custom, ThermoFisher, Massachusetts, US). Frozen exosome suspension was thawed and kept on ice for sample preparation following the manufacturer's instruction. The luminescent signal was detected using LuminexTM 100/200^TM system with xPONENT 3.1 software.

Hoang D.H., Nguyen T.D., Nguyen H.P., Nguyen X.H., Do P.T., Dang V.D., Dam P.T., Bui H.T., Trinh M.Q., Vu D.M., Hoang N.T., Thanh L.N, & Than U.T. (2020). Differential Wound Healing Capacity of Mesenchymal Stem Cell-Derived Exosomes Originated From Bone Marrow, Adipose Tissue and Umbilical Cord Under Serum- and Xeno-Free Condition. Frontiers in Molecular Biosciences, 7, 119.

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