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1260 series chromatograph

Manufactured by Agilent Technologies

The Agilent 1260 Series Chromatograph is a high-performance liquid chromatography (HPLC) system designed for reliable and efficient separation, detection, and quantification of a wide range of chemical compounds. It features a modular design that allows for customization to meet specific analytical requirements.

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2 protocols using 1260 series chromatograph

1

HPLC Analysis of Compounds

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The HPLC system included an Agilent Technologies 1260 series chromatograph (Agilent Technologies, Santa Clara, CA) equipped with a diode array detector (DAD). The separation was achieved using an Agilent SB-C18 (4.6 × 150 mm i.d., 5 μm). The mobile phase was composed of ACN, MeOH, and water (31:31:38, v:v:v), and the analyte was eluted through isocratic elution with a constant flow rate of 1 ml/min. The ultraviolet wavelength was set to 280 nm, and the injection volume was 20 μl.
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2

Quantification of IOP Biodegradation Byproducts

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The IOP concentration in the influent and effluent of both bioreactors was determined by high-performance liquid chromatography (HPLC) using an Agilent Technology 1260 series chromatograph as previously described (Toral-Sánchez et al. 2016 ; Toral-Sánchez et al. 2017 (link)). Identification of biotransformation byproducts of IOP was carried out by HPLC coupled to mass spectroscopy (HPLC-MS) in a Varian ® 500-MS ion trap mass spectrometer as reported in previous work (Toral-Sánchez et al. 2017 (link)). VSS and COD was estimated according to standard methods (Eaton and Franson 2005 ). The ORP and pH of the biological system was monitored using a Thermo Scientific STARA2110 Orion Star A211 benchtop pH meter with electrode stand.
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