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Erg clone epr3864

Manufactured by Roche
Sourced in Switzerland

The ERG (clone EPR3864) is a laboratory equipment product manufactured by Roche. It serves as an antibody used for immunohistochemistry applications. The core function of this product is to detect and identify the presence of the ERG protein in biological samples.

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2 protocols using erg clone epr3864

1

Immunohistochemical Analysis of Tissue Sections

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Freshly cut 4-μm thick formalin-fixed paraffin-embedded tissue sections were subjected to α-SMA (clone 1A4; Roche Diagnostics, Basel, Switzerland) or ERG (clone EPR3864; Roche Diagnostics, Basel, Switzerland) immunostaining on a BenchMark XT autostainer (Roche Diagnostics, Basel, Switzerland). Both antibodies were pre-diluted and used according to manufacturer protocol. The ultraView Universal DAB Detection Kit (Roche Diagnostics, Basel, Switzerland) was used for chromogenic detection.
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2

Comprehensive Immunohistochemical Profiling

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Immunohistochemistry was performed on 4 µm sections cut from the paraffin block using an autostainer (Benchmark ULTRA; Ventana Medical System Inc., Tucson, AZ) using the following antibodies: CD99 (clone 12E7, prediluted, Dako ), epithelial membrane antigen (EMA) (clone E29, 1:50; Dako), Ki67 (clone MIB-1, 1: 100; Dako), desmin (clone DE-R-11, prediluted; Roche), myogenin (clone F5D, 1: 2; Dako), smooth muscle actin alpha (clone 1A4, prediluted; Microm), PS100 (polyclonal, 1: 400; DBS), PHOX2B (clone, NC, 1:200, Finetest), BCOR (clone C-10, 1:50, Cliniscience), BRG1 (clone EPNCIR111A, 1:100, Abcam), ETV4 (clone 16, 1:25, Santa-Cruz), NUT (clone C52B1, 1:100, Ozyme), ERG (clone EPR3864, prediluted, Roche).
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