Thermo betasil c18

An Agilent 5530 Accurate Mass Quadrupole Time Of Flight (QTOF) Liquid Chromatography (LC)/ MS (CA, US) was used to obtain high resolution (+) Electrospray Ionization (ESI) MS data. Alltech Davisil 30–40 um 60 Å C₁₈ bonded silica gel (Grace Davison Discovery Sciences, MD, US) was used to adsorb the sample prior to HPLC separation and for Medium Pressure Liquid Chromatography (MPLC) separations. A Merck Hitachi L7100 pump equipped with a Merck Hitachi L7455 Photodiode Array (PDA) detector (Hitachi Ltd., Tokyo, Japan) were also used for HPLC analysis. HPLC columns used include a Thermo Betasil C₁₈ 5 μm, 100 Å, 150 mm × 21.2 mm, (Thermofisher Scientific, Massachusetts, US) and a Thermo Betasil C₁₈ 5 μm, 100 Å, 250 mm × 10 mm and Phenyl bonded silica HPLC column 5 μm, 100 Å, 250 mm × 21.2 mm (Thermofisher Scientific, Massachusetts, US). Ion exchange chromatography was done using Amberjet 1200H strong acid cation exchanger (Rohm and Haas, Philadelphia, US). The fractions from the HPLC separation were dried using a GeneVac HT-12 centrifugal evaporator (SP Scientific, NY, USA). All solvents used during HPLC and MS were HPLC grade. All solvents used for HPLC and MS were Lab-Scan HPLC grade (RCI Labscan, Bangkok, Thailand), the H₂O being Millipore Milli-Q PF filtered. Trifluoroacetic acid (TFA) was spectroscopy grade from Alfa Aesar (Thermofisher Scientific, NY, US).