Anti glycogen synthase kinase 3β

Anti-Nodal and anti-β-actin antibodies as well as CDDP, doxorubicin (Dox), MG132, and lithium chloride (LiCl) were purchased from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Anti-X-linked inhibitor of apoptosis (XIAP), anti-Bax, anti-Glycogen Synthase Kinase (GSK)-3β, and anti-p27^Kip1 antibodies were bought from BD Biosciences (San Jose, CA, USA). Anti-p21^waf1, anti-Cyclin D1, anti-Bcl2, and anti-Ki-67 antibodies were purchased from Dako (Copenhagen, Denmark). Anti-cleaved caspase-3 and anti-phospho(p) GSK-3β at Ser9 (pGSK-3β) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-Smad2 and anti-pSmad2 at Serine 255 (pSmad2) antibodies were from Abcam (Cambridge, MA, USA). Anti-Cyclin A2 antibody was from Novocastra (Newcastle, UK). Recombinant transforming growth factor (TGF)-β1 was purchased from R&D Systems (Minneapolis, MN, USA).

Anti-LEFTY, anti-Smad2, and anti-phospho(p)-Smad2 at serine 255 (pSmad2) antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-p27^Kip1 and anti-glycogen synthase kinase (GSK)-3β antibodies were bought from BD Biosciences (San Jose, CA, USA). Anti-p21^waf1, anti-cyclin D1, and anti-Ki-67 antibodies were purchased from Dako (Copenhagen, Denmark). Anti-cyclin A2, anti-estrogen receptor α (ERα), and anti-progesterone receptor (PR) antibodies were from Novocastra (Newcastle, UK). Anti-pGSK-3β at Ser9 (pGSK-3β), anti-Akt, anti-pAkt at serine 473 (pAkt), and anti-ubiquitin antibodies were from Cell Signaling Technology (Danvers, MA, USA). Anti-β-actin antibody, nocodazole, 17β-estradiol (E2), medroxyprogesterone 17-acetate (MPA), MG132, and lithium chloride (LiCl) were purchased from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Rapamycin and aphidicolin were obtained from Calbiochem (Cambridge, MA, USA). Recombinant TGF-β1 was purchased from R&D Systems (Minneapolis, MN, USA).