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Triton 0.2

Manufactured by Merck Group
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Sourced in Belgium

Triton 0.2% is a laboratory detergent solution. It is a non-ionic surfactant commonly used in various biochemical and cell culture applications.

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Lab products found in correlation

Phosphate buffered saline (pbs), by Euroclone (1 mentions) Facscalibur, by Beckman Coulter (1 mentions) Cytoflex s, by Beckman Coulter (1 mentions)

Close spelling variants of this product

Triton X-100 0.2% (2 citations) Triton X (0.2%) (2 citations) Triton 2% (2 citations) Triton (168 citations)

4 protocols using triton 0.2

1

Cell Cycle Analysis by Flow Cytometry

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Cells, washed twice with cold 1X PBS (Euroclone), were resuspend in 1% paraformaldehyde for 45 minutes. After that, the same volume 1X PBS with Triton 0,2% (Sigma) was added for 15′ minutes. Cells were then centrifuged and resupended in 250 μl of PBS with 0.1 mg/ml PI (Sigma) and 100 μg/mL RNAse (Sigma), vortexed for 5 seconds and incubated at room temperature for at least 1 hour in the dark. Samples were analyzed by flow cytometry within 1 hour (Becton-Dickinson FACS Calibur or Beckman-Coulter CytoFLEX S). Data were analyzed with the Summit Software 4.3 or CytExpert Software.
Barbarani G., Fugazza C., Barabino S.M, & Ronchi A.E. (2019). SOX6 blocks the proliferation of BCR-ABL1+ and JAK2V617F+ leukemic cells. Scientific Reports, 9, 3388.
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2

Kinetic Assay for Cell Viability Assessment

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Cell viability was assessed by cellular leakage of LDH using a kinetic assay [46 (link)]. At the end of exposure, supernatants were transferred to a new plate and cells were incubated with triton 0.2% (Sigma-Aldrich, Belgium). After 30 min, plates were centrifuged at 1600 g for 10 min. After transfer to a new plate, freshly prepared substrate solution (pyruvate+NADH) was added and the absorbance was measured by a spectrophotometer at 340 nm for 3 min with 15 s interval. Slope was calculated according to the standard curve. Cell viability was calculated as
slope of leakage/slope of lysate+slope of leakage∗100and relative viabilityassample viability/untreated control viability∗100
Murugadoss S., Brassinne F., Sebaihi N., Petry J., Cokic S.M., Van Landuyt K.L., Godderis L., Mast J., Lison D., Hoet P.H, & van den Brule S. (2020). Agglomeration of titanium dioxide nanoparticles increases toxicological responses in vitro and in vivo. Particle and Fibre Toxicology, 17, 10.
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3

Quantifying Cell Viability via LDH Leakage

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Effect on cell viability was assessed by cellular leakage of lactate dehydrogenase (LDH) using a kinetic assay [31 (link)]. At the end of exposure, supernatants were transferred to a new plate and cells were incubated with triton 0.2% (Sigma-Aldrich, Belgium). After 30 min, plates were centrifuged at 1600 g for 10 min. After transfer to a new plate, freshly prepared substrate solution (pyruvate + NADH) was added and the absorbance was measured by a spectrophotometer at 340 nm for 3 min with 15 s interval. Slope was calculated according to the standard curve. Cell viability was calculated as
slope of leakage/slope of lysate+slope of leakage∗100and relative viabilityassample viability/untreated control viability∗100
Murugadoss S., van den Brule S., Brassinne F., Sebaihi N., Mejia J., Lucas S., Petry J., Godderis L., Mast J., Lison D, & Hoet P.H. (2020). Is aggregated synthetic amorphous silica toxicologically relevant?. Particle and Fibre Toxicology, 17, 1.
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4

Evaluating Adenoviral Transduction in SIEA Flaps

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SIEA flaps were infected with Ad.GFP (MOI 10; n = 9) or Ad.TK (MOI 10; n=3) as a non-GFP encoding vector. Flaps were harvested 72 hours, 1 week or 3 weeks following infection (n=3 animals/time point) along with the underlying flap bed and SIEA pedicle. Samples were formalin fixed for 24 hours, embedded in paraffin and sectioned at a thickness of 3 μm. Sections were de-waxed, permeabilized in Triton 0.2% (Sigma Aldrich, Crawley, UK) and blocked for 1 hour in PBS with bovine serum albumin (1%) and fetal calf serum (2%) at room temperature. Sections were incubated with a polyclonal rabbit anti-GFP primary antibody (1:100 in blocking solution) (Abcam, Cambridge, UK) at 4°C overnight. The following day, sections were washed in PBS and incubated with a goat anti-rabbit 488 FITC-conjugated secondary antibody (1:1000 in blocking buffer) (Life Technologies, Paisley, UK) for 1 hour at room temperature and then washed in PBS with 4’,6-diamidino-2-phenylindole (DAPI; 1:50,000). Sections were then washed in PBS and fixed in paraformaldehyde (4%) for 15 minutes. Confocal images were captured on a Leica Microsystems TCS-SP2 confocal. Cell counts were performed at ×400 magnification.
Adenovirally delivered enzyme prodrug therapy with herpes simplex virus-thymidine kinase in composite tissue free flaps shows therapeutic efficacy in rat models of glioma. (2015). Plastic and reconstructive surgery, 135(2), 475-487.
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