Goat anti rabbit igg sab43714

Western blotting was carried out as previously described [28 (link)]. Briefly, proteins were extracted from costal diaphragm tissues using RIPA lysis buffer (R0010, Solarbio Science & Technology Co., Ltd., Beijing, China) before being quantified using a bicinchoninic acid (BCA) protein assay (PC0020, Solarbio) according to the manufacturer’s instructions. The proteins were then separated by SDS–PAGE and transferred to polyvinylidene difluoride (PVDF) membranes, which were incubated overnight at 4 °C with one of the following primary antibodies: anti-TMPRSS13 (JL-T0294, Jianglai, Shanghai, China), anti-UCH-L1 (NBP2-29420, Novus Biologicals, Littleton, CO, USA), anti-CST3 (C8950, United States Biological, Salem, MA, USA) or anti-β-actin (PAB36265, Bioswamp, Wuhan, China). On the following day, the membranes were incubated with goat anti-rabbit IgG (SAB43714, Bioswamp) as the secondary antibody for 1 h at room temperature. Finally, images of the western blots were scanned and analyzed with Tanon GIS version 4.2 (Tanon Technology, Shanghai, China) to quantify the density of the visible bands, with the results normalized relative to those for β-actin.

Human promyelocytic leukemia cells (HL-60) and acute lymphatic leukemia cells (CCRF-CEM) were obtained from the Shanghai Cell Bank of the Chinese Academy of Sciences. DAC (A119533) was purchased from Aladdin (China). Lipofectamine® 2000 (11668-027) was purchased from Invitrogen (China). Opti-MEM (M5650) was purchased from Sigma (USA). The dual luciferase reporter gene assay kit (RG027) was purchased from Beyotime (China). The cell counting kit 8 (CCK8) (C1706) was purchased from Bioswamp (China). The SYBR FAST qPCR Master Mix (KM4101) was purchased from KAPA Biosystems (USA). The oligo DT18/miR-135a-5p RT Primer (3806), recombinant RNase inhibitor (2313A), and PrimeScript II RTase (2690A) were purchased from TAKARA (Japan). BCA protein concentration assay kit (PC0020) was purchased from Solarbio (China), DAB (DA1010) was purchased from Solarbio (China), and all antibodies (anti-Bad PAB32756; anti-Bcl-2 PAB30599; anti-cleaved-caspase-3 MAB37300; anti-GAPDH PAB36269; goat anti-rabbit IgG SAB43714) were purchased from Bioswamp (China).