Cells within the vasculature and tissue were distinguished using an intravascular stain as previously described (35 ). Briefly, mice received 1μg of fluorophore-conjugated rat anti-mouse CD45.2 (clone 104; BioLegend, San Diego, CA) in 200μL of PBS by retroorbital intravenous injection three minutes prior to euthanasia.
Fluorophore conjugated rat anti mouse cd45.2 clone 104
Manufactured by BioLegend
Fluorophore-conjugated rat anti-mouse CD45.2 (clone 104) is a monoclonal antibody that binds to the CD45.2 isoform of the mouse CD45 cell surface antigen. CD45 is a protein tyrosine phosphatase that is expressed on most hematopoietic cells.
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Lab products found in correlation
2 protocols using fluorophore conjugated rat anti mouse cd45.2 clone 104
1
Intravascular Staining of Leukocytes
2
Fluorophore-Conjugated CD45.2 Labeling
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Three minutes prior to euthanasia, mice were administered 1 μg of fluorophore-conjugated rat anti-mouse CD45.2 (clone 104; BioLegend, San Diego, CA) in 200 μL of PBS by retroorbital intravenous injection as previously described (34 (link)).
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