PBMC-derived EVs were absorbed onto glow discharged carbon coated grids (G2400C from Plano) for 2 min. The solution was blotted of and negatively stained with 4% ammonium molybdate (Sigma-Aldrich) solution for 30 s. Micrographs were recorded with a Jeol JEM 100CX electron microscope at 100 kV onto Kodak SO163 film. Negatives were digitized with a Hasselblad Flextight × 5 scanner at 3000 dpi, resulting in a pixel size of 0.25 nm/px. For visualization images were binned to 1 nm/px.
Jem 100cx electron microscope
Manufactured by Kodak
The JEM 100CX electron microscope is a high-performance instrument designed for advanced materials analysis. It features a high-resolution electron beam and advanced imaging capabilities for detailed sample examination. The core function of the JEM 100CX is to provide users with a powerful tool for visualizing and analyzing the microstructure of materials at the nanoscale level.
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Lab products found in correlation
2 protocols using jem 100cx electron microscope
1
EV Visualization Using Electron Microscopy
2
Exosome Visualization via Negative Staining
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BHY and FaDu exosomes (isolated from 3 ml conditioned medium) were absorbed onto glow discharged carbon coated grids (G2400C from Plano) for 2 minutes. The solution was blotted of and negatively stained with 4% ammonium molybdate (Sigma-Aldrich) solution for 30 seconds. Micrographs were recorded with a Jeol JEM 100CX electron microscope at 100 kV onto Kodak SO163 film. Negatives were digitized with a Hasselblad Flextight × 5 scanner at 3000 dpi, resulting in a pixel size of 0.25 nm/px. For visualization images were binned to 1 nm/px.
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