Whatman nucleopore track etched membranes

Manufactured by Cytiva

Sourced in Canada

Whatman® Nucleopore track-etched membranes are porous filtration membranes used for size-selective separation and isolation of particles, cells, and macromolecules. These membranes are produced by a track-etching process that creates uniform, cylindrical pores of precise and consistent size.

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Lab products found in correlation

Mpes microkros module, by Repligen (1 mentions)

Close spelling variants of this product

Nucleopore track-etched membrane Nucleopore®-Track Etched Nucleopore membrane Whatman membrane Nucleopore

2 protocols using whatman nucleopore track etched membranes

Anionic T Helper Liposome Generation

Cited 1 time

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The generation of anionic T helper liposomes by thin-film hydration with a lipid composition of 45 mol% DSPC, 40 mol% cholesterol and 15 mol% DSPG was previously described in detail [24 (link)]. OT2 peptide solution was used for hydration of the lipid film. Subsequently, extrusion was performed at 55 °C to minimize peptide loss using a LIPEX™ Extruder (Northern Lipids Inc., Burnaby, BC, Canada) with Whatman^® Nucleopore track-etched membranes (5 cycles with 400 nm pore size, then 5–10 cycles with 200 nm or 100 nm pore sizes). Finally, the nanoparticle suspension was sterile-filtered (0.2 µm) and stored at 4 °C. Non-encapsulated peptides were removed by tangential flow filtration using a 100 kDa mPES MicroKros^® module (Repligen, Waltham, MA, USA) with a surface area of 20 cm² [24 (link)].

Damm D., Suleiman E., Theobald H., Wagner J.T., Batzoni M., Ahlfeld (née Kohlhauser) B., Walkenfort B., Albrecht J.C., Ingale J., Yang L., Hasenberg M., Wyatt R.T., Vorauer-Uhl K., Überla K, & Temchura V. (2022). Design and Functional Characterization of HIV-1 Envelope Protein-Coupled T Helper Liposomes. Pharmaceutics, 14(7), 1385.

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Vesicle Preparation for Membrane Transport

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Chloroform phospholipid stocks were mixed and dried under a vacuum overnight to form dried lipid films. Films were then rehydrated in the appropriate buffer [see below; acceptor vesicles, 10 mM TRIS, 100 mM NaCl, and 0.5 mM EDTA (pH 7.6); donor vesicles, the same buffer with 20 wt % sucrose], sonicated for ~5 min, and subjected to 12 freeze–thaw cycles. Acceptor vesicles were extruded through 100 nm Whatman nucleopore track-etched membranes 21 times to form large unilamellar vesicles. Donor multilamellar vesicles were diluted 20-fold with buffer and centrifuged at 20000g for 30 min at 30 °C, resulting in vesicle pellets. The supernatant was decanted, and pellets were resuspended in buffer to a total lipid concentration of 15 mM.

Povilaitis S.C, & Webb L.J. (2023). Leaflet-Dependent Effect of Anionic Lipids on Membrane Insertion by Cationic Cell-Penetrating Peptides. The journal of physical chemistry letters, 14(25), 5841-5849.

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