Horse radish peroxidase linked anti rabbit secondary antibodies

[8-¹⁴C]-IMP and [8-¹⁴C]-inosine were purchased from Moravek Biochemicals and Radiochemicals (Brea, CA, USA); DTT, ATP, ADP, AMP, MgCl₂ 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), 3,3′,5,5′-tetramethylbenzidine (TMB), STOP solution, primary antibody against NT5C2 and 2-deoxyglucose (2-dG) were from Sigma (Milano, Italy) and polyethyleneimine (PEI)-cellulose precoated thin-layer plastic sheets were obtained from Merck (Darmstadt, Germany) and prewashed once with 10% NaCl and three times with deionized water before use. DE-81 chromatographic paper was from Whatman (Madstone, UK), horse radish peroxidase (HRP)-linked anti-rabbit secondary antibodies were from Cell Signaling (Danvers, MA, USA) and scintillation liquid Optiphase Hisafe 2 was from Beckman (Brea, CA, USA). Acrylamide and TEMED were from Bio-Rad (Segrate, Milan); penicillin/streptomycin; glutamine and fetal bovine serum (FBS), RPMI and DMEM media were from Euroclone (Pero, Milan) and a Duolink kit for the proximity ligation assay (PLA) assay was from Olink Bioscience (St. Louis, MO, USA). The human lung carcinoma (A549) and the human astrocytoma (ADF) cell lines were purchased from ATCC (ATCC^® CCL-185TM) and routinely tested for Mycoplasma contamination by PCR. All other reagents were of reagent grade.

Commercially available RE was purchased from Vitiva (Markovci, Slovenia). CA and CL were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Acetonitrile and formic acid were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Butyl paraben was purchased from Acros Organics (Fair Lawn, NJ, USA). Mouse plasma was purchased from Innovative Research Inc. (Novi, MI, USA). Dextran sodium sulfate was purchased from Alfa Aesar (Haverhill, MA, USA). RNAlater was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fluorescein isothiocyanate (FITC)–dextran (40 kDa) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Sestrin 2, zonula occludens-1 (ZO-1), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) rabbit polyclonal primary antibodies were purchased from Proteintech (Rosemont, IL, USA). Horseradish peroxidase (HRP)-linked anti-rabbit secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).