The samples used were natural mackerel produced in Nagasaki prefecture, Japan and farmed mackerel produced in Karatsu, Japan. General reagents were purchased from FUJIFILM Wako Pure Chemical Co., (Osaka, Japan) . Trifluoroacetic acid was purchased from Watanabe Chemical Industries, Ltd. (Hiroshima, Japan) . The labeling reagents 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) , fluorescein sodium salt, Trolox ® , 2,2′ -azobis (2-methyl propionamidine) dihydrochloride (AAPH) , and ABTS TM were purchased from Sigma-Aldrich (St. Louis, MO, USA) .
Abts
Manufactured by Merck Group
Sourced in United States, Germany
ABTS TM is a laboratory reagent used in colorimetric assays. It serves as a substrate for the detection and quantification of various enzymes, such as horseradish peroxidase (HRP) and certain oxidases. The product provides a sensitive means of measuring enzymatic activity through the generation of a colored reaction product.
Automatically generated - may contain errors
Lab products found in correlation
Rnalater, by Thermo Fisher Scientific (2 mentions)
Fluorescein sodium salt, by Merck Group (1 mentions)
General reagents, by Fujifilm (1 mentions)
4 fluoro 7 nitro 2 1 3 benzoxadiazole nbd f, by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
2 2 azobis 2 methylpropionamidine dihydrochloride aaph, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Merck Group (1 mentions)
Glutathione reductase, by Merck Group (1 mentions)
O dianisidine dihydrochloride, by Merck Group (1 mentions)
Nadph, by Merck Group (1 mentions)
Trichloroacetic acid tca, by Merck Group (1 mentions)
2 protocols using abts
1
Mackerel Fatty Acid Profiling
2
Comprehensive Oxidative Stress Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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o-Dianisidine dihydrochloride, ferrous ammonium sulfate, xylene orange, 2,4-di-nitrophenylhydrazine, 1,1,3,3 Tetraethoxypropane, ABTSTM, NADPH, glutathione reductase (GR), and Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were purchased from Sigma-Aldrich, Darmstadt, Germany. Trichloroacetic acid (TCA) and 5,5′-Dithiobis (2-nitrobenzoic acid) were obtained from Merck, Darmstadt, Germany. Thiobarbituric acid (TBA) was purchased from VWR, Dietikon, Switzerland. All other chemicals were of analytical grade. Pierce Protease inhibitor cocktail (A32953), Trizol reagent, RNAlater, and bicinchoninic acid assay (BCA assay) reagents were obtained from Thermo Fisher Scientific, Rockford, IL, USA. Lipid determination kits (Innoline Merck TCH, Innoline Merck Triglycerides, Darmstadt, Germany and Spinreact HDLc-D, St. Esteve de Bas, Girona, Spain), ELISA Kit for 8-OHdG (MyBioSource; MBS267161, San Diego, CA, USA), and antibodies used for immunoblotting are mentioned in the respective sections.
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