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Collagenase 2 and 4 solution

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Collagenase II and IV solution is a mixture of enzymes used for the dissociation of tissue samples. It contains collagenase type II and type IV, which help to break down the extracellular matrix and release cells from the tissue.

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Lab products found in correlation

Dnase, by Roche (2 mentions)

Spelling variants of this product

Collagenase IV (716 citations) Collagenase (527 citations) Collagenase II (403 citations) Collagenase solution (11 citations) Collagenase II solution (9 citations) Collagenase IV solution (5 citations) Collagenase II and IV (2 citations)

2 protocols using collagenase 2 and 4 solution

1

Isolation and Analysis of Pancreatic Islet Cells

Cited 1 time
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Pancreatic islet tumors of RIP-Tag2 mice perfused with PBS after anesthesia and were removed, weighed, and digested in collagenase II and IV solution (625U/ml, Gibco) with DNase (60 U/ml, Roche), followed by erythrocyte lysis (24 (link)). Dissociated cells were counted and stained for flow cytometric assessment of CD4+, CD8+, Foxp3+, and NK1.1+ cells by FACS (BD LSR Fortessa™) and analyzed with FlowJo software (8.8.6 and X) (details in Supplementary Methods).
Inoue T., Byrne T., Inoue M., Tait M.E., Wall P., Wang A., Dermyer M.R., Laklai H., Binder J.J., Lees C., Hollingsworth R., Maruri-Avidal L., Kirn D.H, & McDonald D.M. (2021). Oncolytic Vaccinia Virus Gene Modification and Cytokine Expression Effects on Tumor Infection, Immune Response, and Killing. Molecular Cancer Therapeutics, 20(8), 1481-1494.
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2

Pancreatic Islet Tumor Immunophenotyping

Cited 1 time
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Pancreatic islet tumors of RIP-Tag2 mice perfused with PBS after anesthesia and were removed, weighed, and digested in collagenase-II and -IV solution (625U/mL, Gibco) with DNase (60 U/mL, Roche), followed by erythrocyte lysis (24 (link)). Dissociated cells were counted and stained for flow cytometric assessment of CD4+, CD8+, Foxp3+, and NK1.1+ cells by FACS (BD LSR Fortessa) and analyzed with FlowJo software (8.8.6 and X; details in Supplementary Methods).
Inoue T., Byrne T., Inoue M., Tait M.E., Wall P., Wang A., Dermyer M.R., Laklai H., Binder J.J., Lees C., Hollingsworth R., Maruri-Avidal L., Kirn D.H, & McDonald D.M. (2021). Oncolytic Vaccinia Virus Gene Modification and Cytokine Expression Effects on Tumor Infection, Immune Response, and Killing. Molecular Cancer Therapeutics, 20(8), 1481-1494.
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