Apc conjugated cd90

Manufactured by BioLegend

APC-conjugated CD90 is a fluorescently labeled antibody that binds to the CD90 (Thy-1) surface antigen. CD90 is a glycosylphosphatidylinositol (GPI)-anchored protein expressed on various cell types, including T cells, hematopoietic stem cells, and certain mesenchymal stem cells. The APC (Allophycocyanin) fluorescent dye is conjugated to the CD90 antibody, allowing for the detection and analysis of CD90-positive cells using flow cytometry.

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Lab products found in correlation

Attune nxt, by Thermo Fisher Scientific (2 mentions) Fitc conjugated cd45, by BD (1 mentions) Pe conjugated cd144, by BD (1 mentions) Facsaria 3, by BD (1 mentions) Accuri c6, by BD (1 mentions) Facsaria 2 cell sorter, by BD (1 mentions)

2 protocols using apc conjugated cd90

Cell Isolation and Flow Cytometry

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At desired time points, nonadherent and adherent cells were harvested separately by aspiration and dissociation with 0.5 mM EDTA for 15 min, respectively. The cells were then filtered through 40 μm strainers, pelleted, and stained with antibodies in PBE buffer (PBS + 0.5% BSA + 2 mM EDTA). Antibodies of flow cytometry were used as follows: PerCP-conjugated CD34 (BioLegend), FITC-conjugated CD45 (BD Biosciences), and APC-conjugated CD90 (BioLegend), PE-conjugated CD144 (BD Biosciences), and APC-conjugated CD73 (BD Biosciences). Detailed information on these antibodies is provided in Additional file 1: Table S1. All samples were analyzed using BD Accuri™ C6 (BD Biosciences) and Attune™ Nxt (ThermoFisher Scientific). Cell sorting was performed using a FACSAria™ III (BD Biosciences). The data of flow cytometry was analyzed using Flowjo software.

Thongsa-ad U., Wongpan A., Wongkummool W., Chaiwijit P., Uppakara K., Chaiyakitpattana G., Singpant P., Tong-ngam P., Chukhan A., Pabuprappap W., Wongniam S., Suksamrarn A., Hongeng S., Anurathapan U., Kulkeaw K., Tubsuwan A, & Bhukhai K. (2024). Improving hematopoietic differentiation from human induced pluripotent stem cells by the modulation of Hippo signaling with a diarylheptanoid derivative. Stem Cell Research & Therapy, 15, 60.

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Multistage Hematopoietic Cell Immunophenotyping

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hBMSCs, hDPSCs, and hASCs at passages 3, 5, 9, and 11 were detached from flasks when the cells grew to 90% confluency. The cells were collected and resuspended in 1 × DPBS with 1% (v/v) FBS (namely 1 × DPBSF) and filtered through 40 μm cell strainers to prepare a single-cell suspension. Next, the single-cell suspensions were incubated with APC-conjugated CD90 (BioLegend, cat. # 328114) and FITC-conjugated CD105 (BioLegend, cat. # 323204) antibodies at 1:20 dilution for 20 min on ice and 5 min at room temperature. After incubation, the cells were washed with 3 ml 1 × DPBSF to remove unbound antibodies. The supernatants were discarded following centrifugation at 300 × g for 5 min, and the cell pellets were resuspended in 1 × DPBSF at a cell density of 1×10⁶ cells/100 μl and analyzed by a flow cytometer (FACS Aria II cell sorter, BD Biosciences).

Cebolla A., Sousa C, & Lorenzo V.D. (2001). Rational design of a bacterial transcriptional cascade for amplifying gene expression capacity. Nucleic Acids Research, 29(3), 759-766.

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