Igfbp4

Manufactured by Abcam

Sourced in United Kingdom

IGFBP4 is a laboratory reagent used for research purposes. It is a member of the insulin-like growth factor binding protein (IGFBP) family, which plays a role in regulating the bioavailability and activity of insulin-like growth factors. The core function of IGFBP4 is to bind and modulate the effects of insulin-like growth factors.

Automatically generated - may contain errors

Lab products found in correlation

Col1a2, by Abcam (1 mentions) Nupage bis tris gradient mini gels, by Thermo Fisher Scientific (1 mentions) Col18a1, by Merck Group (1 mentions) Bca assay, by Bio-Rad (1 mentions) Quantity one software, by Bio-Rad (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Ex 527, by R&D Systems (1 mentions) Sb203580, by Enzo Life Sciences (1 mentions) Sp600125, by Enzo Life Sciences (1 mentions) Rolipram, by Enzo Life Sciences (1 mentions) A769662, by Santa Cruz Biotechnology (1 mentions) Resveratrol, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-IGFBP4

2 protocols using igfbp4

Quantitative Protein Analysis in CSF

Check if the same lab product or an alternative is used in the 5 most similar protocols

For each CSF sample, protein concentrations were measured with the bicinchoninic acid (BCA) assay (Bio-Rad Laboratories Srl, MI, IT). The protein content of the samples ranged from 0.25 μg/μl to 1.25 μg/μl, and 20 μg of each sample were loaded on 4-12% NuPAGE Bis-Tris gradient mini gels (Life Technologies, Grand Island, NY).
Protein electroblotting was performed following standard procedures, using antibodies for IGSF8, ITIH4, PCOLCE, FGA, FGG, GFRalpha2 and COL18A1 from Sigma-Aldrich Ltd (The Old Brickyard, New Road, Gillingham, Dorset SP8 4XT); for COL1A2, HRG, IGFBP4, MGP, NPDC1 and ribonuclease A from Abcam 330 (Cambridge Science Park, Cambridge CB4 0FL, UK); for selenoprotein P from Santa Cruz (10410 Finnell Street Dallas, Texas 75220 USA). WB bands were quantified with Quantity One software (Bio-Rad, USA) in terms of density (total intensity/area of volume).

Spreafico F., Bongarzone I., Pizzamiglio S., Magni R., Taverna E., De Bortoli M., Ciniselli C.M., Barzanò E., Biassoni V., Luchini A., Liotta L.A., Zhou W., Signore M., Verderio P, & Massimino M. (2017). Proteomic analysis of cerebrospinal fluid from children with central nervous system tumors identifies candidate proteins relating to tumor metastatic spread. Oncotarget, 8(28), 46177-46190.

+ Open protocol

+ Expand

Signaling Pathway Modulation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumor necrosis factor alpha (TNF-α) was purchased from Research Diagnostics Inc. (Flanders, NJ). Interleukin 1- β (IL1-β), interleukin- 6 (IL-6), IL-6 soluble receptor and EX 527 were purchased from R&D Systems (Minneapolis, MN). Antibodies against total and phosphorylated extracellular-regulated protein kinase (ERK) 1/2, c-Jun NH2-terminal kinase (JNK), p38 mitogen-activated kinase, nuclear factor κB (NFκB) [including inhibitory κB (IκB) α and β], phospho-Hsp27, and IGFBP-4 were purchased from Abcam (Cambridge, MA). Antibodies against total and phosphorylated AKT were purchased from Novus Biologicals (Littleton, CO). SB203580, SP600125, rolipram and splitomycin were purchased from Enzo Life Sciences (Farmingdale, NY). Bay 11-7082 was purchased from Calbiochem (San Diego, CA). Resveratrol was purchased from Sigma-Aldrich (St Louis, MO). Reagents for SDS-PAGE, mini-gels and blocking buffer were purchased from Bio-Rad Laboratories (Richmond, CA) and tissue culture supplements and fetal bovine serum were purchased from Life Technologies (Grand Island, NY). A769662 was purchased from Santa Cruz Biotechnology (Dallas, TX).

Davidge-Pitts C., Escande C.J, & Conover C.A. (2014). Preferential Expression of PAPP-A in Human Preadipocytes from Omental Fat. The Journal of endocrinology, 222(1), 87-97.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!