Acetylated histone 3

All cells cultured in DMEM with 10% FBS (Invitrogen, Carlsbad CA) and 100 μ/mL Penicillin/Streptomycin (Invitrogen) unless otherwise noted. NIH 3T3 cells (ATC#CRL-1658) were obtained from the ATTC. HFF-1 cells (ATCC#SCRC-1041) were obtained from the ATTC. Mouse fibroblasts were generated as described (Gasser et al., 2005 (link)) and provided by Pr D Raulet. MCA-205 were received from Pr. L. Linear (UCSF). All cell lines tested negative for mycoplasma as described by Young et al. (2010) (link). Mithramycin A and Butyrate were purchased from Sigma (St. Louis, MO). RGFP966 was purchased from Seleckchem (Houston, TX). Antibodies recognizing Sp1 (product # 07–645), Acetylated Histone 3 (product # 06–599) were purchased from Millipore (Billerica, MA). Antibodies against histone 3 (clone D1H2), HDAC3 (Clone 7G685), and antibody recognizing CKII substrate (#8738) were purchased from Cell Signaling Technologies (Danvers MA). Antibody recognizing CK2β (Product PA5-27416) was purchased from Thermo Fisher (Waltham, MA). Antibody against Sp3 (D-20) was purchased from Santa Cruz Biotechnology. Plasmid encoding HSV-1 ICP0 was provided by the Knipe Lab (Harvard). Plasmid encoding HCMV IE1 was provided by the Weinberger Lab (UCSF).

DW22, with purity more than 98%, was synthesized in Medicine Chemistry Laboratory at Shenyang Pharmaceutical University (see Figure 2A). Bexarotene and SAHA were obtained from Sigma, USA. These agents were dissolved in DMSO to 100 mM and stored at −20°C. Before treatment, the stock solution is diluted to different concentrations. The final concentration of DMSO in cultures is 0.1% (v/v) or less. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and Propidium idodide (PI) were purchased from Sigma, USA. The primary antibodies against RXRα, HDAC1, Histone 3, Histone 4, p21 ^WAF1/CIP1, PARP, Caspase-3, KLF4, VEGF, and β-actin were got from Cell Signaling Technology (Danvers, MA). The primary antibodies against Ki67, CD31, specific RXRα siRNA, and control siRNA duplexes were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). The primary antibodies against acetylated Histone 3 and acetylated Histone 4 were purchased from Millipore, USA. The HDAC1 Silencer Select Validated siRNA was got from Life technologies, USA.