Log In Sign up
The largest database of trusted experimental protocols

Pcdna3.1

Manufactured by Thermo Fisher Scientific
Visit Supplier

PcDNA3.1 (þ) is a plasmid vector commonly used in molecular biology research. It is designed for high-level, transient expression of recombinant proteins in a variety of mammalian cell lines. The vector contains a cytomegalovirus (CMV) promoter, which drives the expression of the inserted gene of interest, and a neomycin resistance gene for selection of stable cell lines.

Automatically generated - may contain errors

Lab products found in correlation

Starmut site directed mutagenesis kit, by GenStar (1 mentions) Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

PcDNA3.1-EGFP vector PcDNA3.1/myc-His B PcDNA3.1 Directional TOPO Expression Kit PcDNA3.1(+) (V790-20) PcDNA3.1/V5-His/TOPO DNA vector PCDNA3.1 Directional TOPO Expression vector PcDNA3.1 empty vector (pcDNA; PcDNA3.1/CT-GFP-TOPO PCDNA3.1-Neo(+) expression vector Control pCDNA3.1 empty plasmid

2 protocols using pcdna3.1

1

Cloning and Mutagenesis of AARS2 cDNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human AARS2 (Alanyl-tRNA synthetase 2) gene (Accession no: NM_020745) cDNA clone plasmid was obtained from Vigene Biosciences Inc. We cloned the AARS2 full coding region cDNA into pcDNA3.1 (þ) (Invitrogen). The StarMut Sitedirected Mutagenesis Kit (GenStar) was used to construct the mutant expression vector.
Zhou Y., Chen B., Li L., Pan H., Liu B., Li T., Wang R., Ma X., Wang B, & Cao Y. (2019). Novel alanyl-tRNA synthetase 2 (AARS2) homozygous mutation in a consanguineous Chinese family with premature ovarian insufficiency. Fertility and sterility, 112(3).
+ Open protocol
+ Expand
2

Overexpression of b4 Integrin in CFPAC-1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human b4 integrin full-coding cDNA was amplified by reverse transcription-PCR and inserted into pcDNA 3.1 ( þ ) (Invitrogen). CFPAC-1 cells were transfected with the plasmid. Lipofectamine LTX (Invitrogen) and stable transfectants were selected with G418 (Invitrogen), according to the manufacturer's instructions.
Masugi Y., Yamazaki K., Emoto K., Effendi K., Tsujikawa H., Kitago M., Itano O., Kitagawa Y, & Sakamoto M. (2015). Upregulation of integrin β4 promotes epithelial-mesenchymal transition and is a novel prognostic marker in pancreatic ductal adenocarcinoma. Laboratory investigation; a journal of technical methods and pathology, 95(3).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!