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Mab1594

Manufactured by Merck Group
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MAB1594 is a monoclonal antibody product manufactured by Merck Group. It is intended for use in laboratory research applications.

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Lab products found in correlation

Nupage lds sample buffer, by Thermo Fisher Scientific (2 mentions) Chemiluminescence reagent, by PerkinElmer (2 mentions) Costar spin x centrifuge tube filter, by Corning (2 mentions) Ab113691, by Abcam (2 mentions) Ab175402, by Abcam (2 mentions) Ab124680, by Abcam (2 mentions) Ls c197243, by LifeSpan BioSciences (2 mentions) Stainless steel beads for tissue homogenization, by Next Advance (2 mentions)

2 protocols using mab1594

1

Frataxin Protein Detection Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Four protein standards were used in this study. Cynomolgus monkey mature frataxin (aa 81–210) was purchased from LifeSpan BioSciences (LS-G21788). Mouse recombinant intermediate form of frataxin (aa 41–207) was obtained from LifeSpan BioSciences (LS-G14956). Human and mouse mature frataxin were prepared using a protocol described previously (Guo et al. Anal. Chem.). Five antibodies were tested here, including AB113691, AB175402, AB124680 obtained from Abcam (Cambridge, MA), MAB1594 from Millipore-Sigma (Billerica, MA), and LS-C197243 from LifeSpan BioSciences (Seattle, WA). Anti-rabbit HRP and anti-mouse HRP were obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX). Stainless steel beads for tissue homogenization were purchased from Next Advance (Troy, NY). NuPAGE™ LDS sample buffer was from Thermo Scientific (Waltham, MA) and chemiluminescence reagent (NEL103E001) was purchased from Perkin Elmer (Waltham, WA). Costar® Spin-X centrifuge tube filters (0.22 μm cellulose acetate) were obtained from Corning (Corning, NY).
Weng L., Wang Q., Yu S., Yang X., Lynch D.R., Mesaros C, & Blair I.A. (2019). Evaluation of antibodies for western blot analysis of frataxin protein isoforms. Journal of immunological methods, 474, 112629.
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2

Frataxin Protein Detection Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Four protein standards were used in this study. Cynomolgus monkey mature frataxin (aa 81–210) was purchased from LifeSpan BioSciences (LS-G21788). Mouse recombinant intermediate form of frataxin (aa 41–207) was obtained from LifeSpan BioSciences (LS-G14956). Human and mouse mature frataxin were prepared using a protocol described previously (Guo et al. Anal. Chem.). Five antibodies were tested here, including AB113691, AB175402, AB124680 obtained from Abcam (Cambridge, MA), MAB1594 from Millipore-Sigma (Billerica, MA), and LS-C197243 from LifeSpan BioSciences (Seattle, WA). Anti-rabbit HRP and anti-mouse HRP were obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX). Stainless steel beads for tissue homogenization were purchased from Next Advance (Troy, NY). NuPAGE™ LDS sample buffer was from Thermo Scientific (Waltham, MA) and chemiluminescence reagent (NEL103E001) was purchased from Perkin Elmer (Waltham, WA). Costar® Spin-X centrifuge tube filters (0.22 μm cellulose acetate) were obtained from Corning (Corning, NY).
Weng L., Wang Q., Yu S., Yang X., Lynch D.R., Mesaros C, & Blair I.A. (2019). Evaluation of antibodies for western blot analysis of frataxin protein isoforms. Journal of immunological methods, 474, 112629.
+ Open protocol
+ Expand

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