Mg132

Manufactured by AG Scientific

Sourced in United States

MG132 is a proteasome inhibitor that blocks the activity of the 26S proteasome, a large protein complex that is responsible for the degradation of most cellular proteins. It is commonly used in research applications to study the role of the proteasome in various cellular processes.

Automatically generated - may contain errors

Lab products found in correlation

Cycloheximide (chx), by AG Scientific (7 mentions) Anti actin, by Merck Group (3 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Anti ha, by Merck Group (2 mentions) Chloroquine, by AG Scientific (2 mentions) Anti ciap 1, by Santa Cruz Biotechnology (2 mentions) Dimethyl sulfoxide (dmso), by AG Scientific (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Anti traf6, by Santa Cruz Biotechnology (2 mentions) Anti myc, by Roche (2 mentions) Anti ha, by Roche (2 mentions) Anti flag m2, by Merck Group (2 mentions) Lipofectamine ltx, by Thermo Fisher Scientific (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Anti flag monoclonal antibody, by Merck Group (2 mentions) Cycloheximide (chx), by Cell Signaling Technology (1 mentions) Rad001, by MedChemExpress (1 mentions) Control sirna sc 37007, by Santa Cruz Biotechnology (1 mentions) Anti phospho p70s6k t389, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

MG132 (M-1157) (3 citations)

28 protocols using mg132

Inhibitors for DNA Damage Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

A list of all antibodies used in this study can be found in Supplementary Table 1. The following reagents were used: ATM inhibitor KU59933 (Tocris), ATR inhibitor VE-831, DNA-PKs inhibitor KU57788 (NU7441), PARP inhibitor PJ34 (Selleckchem, Houston, TX, USA), SUMO inhibitor 2D-08 (Sigma, St. Louis, MO, USA), MG132, cycloheximide, and dimethyl sulfoxide (AG Scientific, San Diego, CA, USA).

Ha G.H., Ji J.H., Chae S., Park J., Kim S., Lee J.K., Kim Y., Min S., Park J.M., Kang T.H., Lee H., Cho H, & Lee C.W. (2019). Pellino1 regulates reversible ATM activation via NBS1 ubiquitination at DNA double-strand breaks. Nature Communications, 10, 1577.

+ Open protocol

+ Expand

Inhibition of Protein Synthesis and Degradation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cycloheximide (CHX) was purchased from A.G. Scientific, dissolved in ultra-pure water and used at final concentration of 50 µg/ml. MG132 was purchased from A.G. Scientific, dissolved in DMSO and utilized at final concentrations specified in figure legends.

Blount J.R., Tsou W.L., Ristic G., Burr A.A., Ouyang M., Galante H., Scaglione K.M, & Todi S.V. (2014). Ubiquitin-Binding Site 2 of ataxin-3 prevents its proteasomal degradation by interacting with Rad23. Nature communications, 5, 4638.

+ Open protocol

+ Expand

Flg22 and MG132 Protoplast Treatments

Check if the same lab product or an alternative is used in the 5 most similar protocols

The concentration of flg22 used in this study is 0.1 μM (for the treatment of protoplasts and seedlings) and 0.5 μM (for callose deposition assay). For the seedling treatments, the concentration of MG132 (AG Scientific #99533–80-9) is 2 μM.

Kong L., Feng B., Yan Y., Zhang C., Kim J.H., Xu L., Rack J.G., Wang Y., Jang J.C., Ahel I., Shan L, & He P. (2021). Noncanonical mono(ADP-ribosyl)ation of zinc finger SZF proteins counteracts ubiquitination for protein homeostasis in plant immunity. Molecular cell, 81(22), 4591-4604.e8.

+ Open protocol

+ Expand

Palmitic Acid Signaling Pathway Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Palmitic acid (C16:0, PA) (Sigma, Burlington, MA, USA) was added to the cell culture medium as a PA-BSA complex, as described[8 (link)]. mTOR inhibitor RAD001 (20 nM, 16h) and S6K inhibitor NaSal (10 mM, 16h) were purchased from MedChemExpress (Monmouth Junction, NJ, USA) and Selleck (Houston, TX, USA), respectively. The proteasome inhibitor MG132 (20 μM, 6h) was purchased from A.G Scientific (San Diego, CA, USA). The lysosomal inhibitor leupeptin (50 μM, 16h) was purchased from Sigma (Burlington, MA, USA). The GSK3β inhibitor TWS119 (10 μM, 16h) was obtained from Santa Cruze (Dallas, Texas, USA). Cycloheximide (50 μM) was purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-PTEN, anti-Phospho-PTEN (Ser380), anti-p70S6K, anti-Phospho-p70S6K (T389) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Phospho-PTEN (Thr366) antibody was purchased from Abcam (Cambridge, MA, USA). Anti-vinculin, anti-HA, anti-WWP2 antibodies and PTEN-siRNA (EHU106441) were purchased from Sigma (Burlington, MA, USA). WWP2-siRNA (sc-40362) and control-siRNA (sc-37007) were obtained from Santa Cruze (Dallas, Texas, USA). S6K and mTOR inhibitors were added to cells for 1 h before PA treatment.

Massuda E.S., Dunphy E.J., Redman R.A., Schreiber J.J., Nauta L.E., Barr F.G., Maxwell I.H, & Cripe T.P. (1997). Regulated expression of the diphtheria toxin A chain by a tumor-specific chimeric transcription factor results in selective toxicity for alveolar rhabdomyosarcoma cells. Proceedings of the National Academy of Sciences of the United States of America, 94(26), 14701-14706.

+ Open protocol

+ Expand

Immunoblotting and Immunoprecipitation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunoblotting, phosphate-buffered saline-resuspended cell lysates were mixed with 2X SDS sample buffer in a 1:1 ratio and boiled for 10 min. For immunoprecipitation, cells were lysed in a buffer containing 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM ethylenediaminetetraacetic acid, 0.5% Triton X-100, and a protease inhibitor cocktail (Roche, Switzerland). Proteins from cell extracts were sequentially immunoprecipitated with anti-Flag M2 agarose (A2220; Sigma-Aldrich) overnight at 4°C and Protein G-Sepharose (GE Healthcare) for 2 h at 4°C. Samples were mixed with the 2X SDS sample buffer in a 1:1 ratio and boiled for 5 min. Immunoprecipitated proteins were analyzed by western blotting using anti-HA (12013819001; Sigma-Aldrich), anti-Flag (F3165; Sigma-Aldrich), and anti-β-actin (A300-491A; Bethyl Laboratories, USA). Proteins were detected using enhanced chemiluminescence reagents (GE Healthcare) according to the manufacturer’s instructions. MG132 was purchased from A.G. Scientific (M-1157).

Lee H.S., Kim M.W., Jin K.S., Shin H.C., Kim W.K., Lee S.C., Kim S.J., Lee E.W, & Ku B. (2021). Molecular Analysis of the Interaction between Human PTPN21 and the Oncoprotein E7 from Human Papillomavirus Genotype 18. Molecules and Cells, 44(1), 26-37.

+ Open protocol

+ Expand

Analyzing Ubiquitinated Protein Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293T cells transfected with His-ubiquitin and tagged plasmids as indicated were synchronized by treatment with 100 ng/mL nocodazole for 18 h followed by treatment with 10 μM MG132 (A.G. Scientific. Inc.) for 4 h. The cells were lysed with Urea lysis buffer (8 M Urea, 0.3 M NaCl, 50 mM Na₂HPO_4, 50 mM Tris-HCl, 1 mM phenylmethylsulfonyl fluoride (PMSF), 10 mM Imidazole, pH 8) and sonicated. About 3–5 mg of lysates were incubated with Ni-NTA agarose (Qiagen) for 6 h at 4 °C. The beads were washed with Urea washing buffer (8 M Urea, 0.3 M NaCl, 50 mM Na₂HPO_4, 50 mM Tris-HCl, 1 mM PMSF, 20 mM Imidazole, pH 8) and eluted in 6 × SDS sample buffer. The samples were detected by western blot analysis. Full blot images used in this study are provided in Supplementary Fig. 7.

Park J., Kwon M.S., Kim E.E., Lee H, & Song E.J. (2018). USP35 regulates mitotic progression by modulating the stability of Aurora B. Nature Communications, 9, 688.

+ Open protocol

+ Expand

Treatment with flg22 and Pep1

Check if the same lab product or an alternative is used in the 5 most similar protocols

The elicitors flg22 and Pep1 were reported previously [48 (link)]. MG132 (AG Scientific #99533-80-9) and K252a (AG Scientific #133407-82-6) were diluted from DMSO stock solutions (2 mM for MG132 and 2 mM for K252a).

Yu X., Xu G., Li B., de Souza Vespoli L., Liu H., Moeder W., Chen S., de Oliveira M.V., de Souza S.A., Shao W., Rodrigues B., Ma Y., Chhajed S., Xue S., Berkowitz G.A., Yoshioka K., He P, & Shan L. (2019). The receptor kinases BAK1/SERK4 regulate Ca2+ channel-mediated cellular homeostasis for cell death containment. Current biology : CB, 29(22), 3778-3790.e8.

+ Open protocol

+ Expand

Mammalian Cell Culture and Transfection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mammalian cells used in this study, including HEK293, HEK293-pre1-HTBH, HEK293-trex-htau40 and C2C12 cells, were grown in DMEM supplemented with 10% FBS, 2 mM glutamine, and 100 units per ml penicillin/streptomycin. Cells were maintained in a humidified incubator with 5% CO₂ at 37 °C. For transfection, cells were treated with 1–2 μg of total plasmid DNA in a 6-well culture plate (>95% confluent or at a density of 10⁶ cells per well) for 36–48 h using Lipofectamine 3000 (Invitrogen). Cell lysates were prepared in RIPA buffer 36–48 h post transfection and were used for immunoblotting. For chase analysis, wild type and α3ΔN cells were treated with 75 μg ml⁻¹ cycloheximide (Enzo Life Sciences) and samples were isolated at chase times 0, 20, 40 and 60 min after 4 h transient MG132 (AG Scientific, San Diego, CA, USA) treatment and vigorous washing with phosphate-buffered saline (PBS). For stable cell line maintenance, transfected cells were cultured with DMEM medium containing 600 mg ml⁻¹ G418 (Santa Cruz) and 10% FBS. Fluorescence images were obtained after cells were extensively rinsed three times with PBS.

Shin S.K., Kim J.H., Lee J.H., Son Y.H., Lee M.W., Kim H.J., Noh S.A., Kim K.P., Kim I.G, & Lee M.J. (2017). Docosahexaenoic acid-mediated protein aggregates may reduce proteasome activity and delay myotube degradation during muscle atrophy in vitro. Experimental & Molecular Medicine, 49(1), e287-.

+ Open protocol

+ Expand

EGF and Alexa Fluor 555-EGF Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols

EGF was purchased from Sigma. Alexa Fluor 555-EGF was obtained from Invitrogen. Cycloheximide and MG132 were purchased from AG scientific (San Diego, CA, USA).

Park M.H., Choi K.Y, & Min D.S. (2015). The pleckstrin homology domain of phospholipase D1 accelerates EGFR endocytosis by increasing the expression of the Rab5 effector, rabaptin-5. Experimental & Molecular Medicine, 47(12), e200-.

+ Open protocol

+ Expand

Preparation and Application of Aβ3(pE)-42 Oligomers

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aβ3(pE)-42 oligomers (Anaspec, #AS‐20,276; Fremont, CA) were prepared according to a previously established protocol [28 (link)]. The lyophilized peptide was dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) to 0.5 mg/ml and the aliquots were stored at − 80 °C. HFIP was evaporated for 24 h at room temperature. The peptide was dissolved in 0.1 M NaOH, diluted in NB medium buffered with 0.1 M HCl, and incubated for 24 h. The oligomers were added directly to cultures at a final concentration of 500 nM [24 (link)]. The neural precursor cell-expressed developmentally down-regulated gene 8 (NEDD8) inhibitor MLN-4924 (#A-1139, Active Biochem, Hong Kong, China) was directly applied to the culture medium at a final concentration of 1 μM [29 ]. MG-132 (#M-1157, AG Scientific, San Diego, CA) was used in cell culture experiments at a final concentration of 20 µM.

Confettura A.D., Cuboni E., Ammar M.R., Jia S., Gomes G.M., Yuanxiang P., Raman R., Li T., Grochowska K.M., Ahrends R., Karpova A., Dityatev A, & Kreutz M.R. (2022). Neddylation-dependent protein degradation is a nexus between synaptic insulin resistance, neuroinflammation and Alzheimer’s disease. Translational Neurodegeneration, 11, 2.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!