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Fetalplex

Manufactured by Gemini Bio
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Sourced in United States, Canada

The FetalPlex is a laboratory instrument designed for the analysis and processing of fetal samples. It is a versatile and reliable tool that enables researchers and clinicians to perform essential procedures on fetal specimens. The core function of the FetalPlex is to provide a controlled and secure environment for the handling and processing of fetal samples, ensuring accurate and consistent results.

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Lab products found in correlation

L glutamine, by Thermo Fisher Scientific (4 mentions) C2c12 myoblast, by American Type Culture Collection (4 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Mlpenicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Dulbecco s modified eagle medium with high glucose, by Thermo Fisher Scientific (2 mentions) Dneasy blood tissue kit, by Qiagen (2 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Lonza (1 mentions) Glutamine, by Gemini Bio (1 mentions) Dulbecco modified eagle medium (dmem), by Lonza (1 mentions) Glutamine, by Lonza (1 mentions) Lapatinib, by Avantor (1 mentions) Doxycycline, by Takara Bio (1 mentions) Erlotinib, by Selleck Chemicals (1 mentions) Epidermal growth factor (egf), by GoldBio (1 mentions) Hb egf, by Merck Group (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Na125i, by PerkinElmer (1 mentions)

27 protocols using fetalplex

1

Establishment and Maintenance of Cell Lines

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293T (obtained from Elliott Kieff, Harvard Medical School), a human cell line transformed by adenovirus 5 and SV40 large T antigen [76 (link)], was cultured in Dulbecco’s modified Eagle’s (Gibco) medium. The “wild-type” LCL, created with an unmodified EBV BACmid, was a generous gift from Fred Wang [47 (link)] and the 721 LCL was obtained from Bill Sugden [77 (link)]. LCLs and P3HR1 ZHT cells [78 (link)], a type II EBV cell line, were cultured in RPMI 1640 (Gibco). Media was supplemented with L-glutamine (Gibco), penicillin-streptomycin (Gibco) and 10–15% FetalPlex (Gemini Bio-Products).
Ohashi M., Holthaus A.M., Calderwood M.A., Lai C.Y., Krastins B., Sarracino D, & Johannsen E. (2015). The EBNA3 Family of Epstein-Barr Virus Nuclear Proteins Associates with the USP46/USP12 Deubiquitination Complexes to Regulate Lymphoblastoid Cell Line Growth. PLoS Pathogens, 11(4), e1004822.
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2

Colon Cancer Cell Culture Protocol

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In vivo studies were conducted using LS-174T cells, a human colon carcinoma cell line, grown inDulbecco’s minimum essential medium (DMEM). The DMEM was supplemented with 1 mM glutamine, 1 mM non-essential amino acids (NEAA) and 10% FetalPlex (Gemini Bioproducts, Inc., West Sacramento, Ca) as detailed elsewhere.24 (link), 25 (link) DMEM, glutamine and NEAA were obtained from Lonza, Walkersville, MD. Cells were harvested when 75–80% confluency was attained.
Milenic D.E., Baidoo K.E., Kim Y.S., Barkley R, & Brechbiel M.W. (2017). Comparative Studies on the Therapeutic Benefit of Targeted α-Particle Radiation Therapy for the Treatment of Disseminated Intraperitoneal Disease. Dalton transactions (Cambridge, England : 2003), 46(42), 14591-14601.
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3

Culturing and Differentiating C2C12 Myoblasts

Cited 2 times
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C2C12 myoblasts were obtained from the American Type Culture Collection (Rockville, MD, USA). C2C12 cells were cultured in Dulbecco’s Minimum Essential Medium (DMEM) containing 10% FetalPlex (Gemini Bio-Products, Woodland, CA) and 1% penicillin/streptomycin. C2C12 cells were passaged by using a 5% trypsin, 0.02% EDTA solution every one to two days. Cells were differentiated for two days by incubating with DMEM containing 2% horse serum and 1% penicillin/streptomycin. Cells were grown in a humidified incubator with 5% CO2 at 37 °C. For some experiments, myotubes were serum-starved by incubation with DMEM with antibiotic but without FetalPlex for three hours. C2C12 cell lines expressing green fluorescent protein (GFP) or short hairpin RNA to reduce ATM expression were described previously [5 (link), 18 (link)].
Spears L.D., Tran A.V., Qin C.Y., Hobbs S.B., Liang Burns C.A., Royer N.K., Zhang Z., Ralston L, & Fisher J.S. (2016). Chloroquine increases phosphorylation of AMPK and Akt in myotubes. Heliyon, 2(3), e00083.
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4

Genomic Editing of HEK293T Cells

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HEK293T cells were maintained in Dulbecco's modified Eagle
medium with high glucose, sodium pyruvate, and L-glutamine (Life Technologies)
supplemented with 10% FetalPlex (Gemini Bio-Products) and 50 units/ml
penicillin-streptomycin (Life Technologies). For transfection, 4 x
105 or 2 x 105 cells were plated into a 12- or 24-well
plate, respectively. One day after plating, the cells were transfected with DNA
with Lipofectamine 2000 (Life Technologies), according to manufacturer's
instructions. For point mutagenesis by TALENs, 400 or 200 ng of each TALEN
vector and 800 or 400 ng of an oligonucleotide donor DNA were transfected per
well in a 12- or 24-well plate, respectively. For point mutagenesis by
CRISPR/Cas, 400 or 200 ng of Cas9 vector, 400 or 200 ng of gRNA vector, and 800
or 400 ng of an oligonucleotide donor DNA were transfected per 12- or 24-well,
respectively. Genomic DNA was extracted from the cells 3 days after transfection
with the DNeasy Blood & Tissue Kit (Qiagen).
Miyaoka Y., Chan A.H., Judge L.M., Yoo J., Huang M., Nguyen T.D., Lizarraga P.P., So P.L, & Conklin B.R. (2014). Isolation of single-base genome-edited human iPS cells without antibiotic selection. Nature methods, 11(3), 291-293.
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5

Genomic Editing of HEK293T Cells

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HEK293T cells were maintained in Dulbecco's modified Eagle
medium with high glucose, sodium pyruvate, and L-glutamine (Life Technologies)
supplemented with 10% FetalPlex (Gemini Bio-Products) and 50 units/ml
penicillin-streptomycin (Life Technologies). For transfection, 4 x
105 or 2 x 105 cells were plated into a 12- or 24-well
plate, respectively. One day after plating, the cells were transfected with DNA
with Lipofectamine 2000 (Life Technologies), according to manufacturer's
instructions. For point mutagenesis by TALENs, 400 or 200 ng of each TALEN
vector and 800 or 400 ng of an oligonucleotide donor DNA were transfected per
well in a 12- or 24-well plate, respectively. For point mutagenesis by
CRISPR/Cas, 400 or 200 ng of Cas9 vector, 400 or 200 ng of gRNA vector, and 800
or 400 ng of an oligonucleotide donor DNA were transfected per 12- or 24-well,
respectively. Genomic DNA was extracted from the cells 3 days after transfection
with the DNeasy Blood & Tissue Kit (Qiagen).
Miyaoka Y., Chan A.H., Judge L.M., Yoo J., Huang M., Nguyen T.D., Lizarraga P.P., So P.L, & Conklin B.R. (2014). Isolation of single-base genome-edited human iPS cells without antibiotic selection. Nature methods, 11(3), 291-293.
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6

EGFR Signaling Pathway Activation Assay

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The CHO-K1 Tet-On cell line, the pBI-Tet vector, and doxycycline were from Clontech. EGF and amphiregulin were from Gold Biotechnology. Epigen, epiregulin, and betacellulin were from Prospec. HB-EGF was from Sigma. TGFα was from Abcam. Erlotinib was from Selleck Chemicals and lapatinib was from VWR. The PY-20 antiphosphotyrosine antibody was from BD Transduction Labs. The site-specific anti-phosphotyrosine antibodies plus the anti-T669 antibody were from Cell Signaling Technology. The phospholipase Cγ, phospho-phospholipase Cγ, Akt, phospho-Akt, and phospho-MAP kinase antibodies were also from Cell Signaling Technology. The MAP kinase antibody was from Transduction Labs. FetalPlex was from Gemini Bioproducts. Cetuximab and pertuzumab were obtained from the Barnes-Jewish Hospital pharmacy. Na125I was from Perkin Elmer. 125I-EGF and 125I-cetuximab were made using the ICl method of Contreras et al (60 ).
Macdonald-Obermann J.L, & Pike L.J. (2024). Extracellular domain mutations of the EGF receptor differentially modulate high-affinity and low-affinity responses to EGF receptor ligands. The Journal of Biological Chemistry, 300(3), 105763.
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7

CHO Cell Lines for Receptor Studies

Cited 1 time
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All experiments were conducted using intact cells or membranes prepared from Chinese hamster ovary (CHO) cells stably transfected with either human cannabinoid type-1 receptors (CHO-hCB1), human cannabinoid type-2 receptors (CHO-hCB2) (Shoemaker et al., 2005 (link)), or human mu opioid receptors (CHO-hMOR) (Seely et al., 2012 (link)). CHO-hCB1 cells were purchased from DiscoverRx Corporation (Fremont, CA, USA) and cultured in HAM’s F-12 K media (ATCC, Manassas, VA, USA). CHO-hCB2 and CHO-hMOR cells were cultured in DMEM (Mediatech Inc., Manassas, VA, USA). All media contained 10% Fetalplex, (Gemini Bioproducts, Sacramento, CA, USA), 0.05 IU/mL penicillin, 50 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA), and 250 μg/mL of Geneticin (G418; Sigma-Aldrich, St. Louis, MO, USA). Cells were cultured in a 37°C humidified incubator with 5% CO2 and harvested with PBS (10 mM)/EDTA (1 mM) when 80% confluent. All cells used were maintained between passages 4–15.
Franks L.N., Ford B.M, & Prather P.L. (2016). Selective Estrogen Receptor Modulators: Cannabinoid Receptor Inverse Agonists with Differential CB1 and CB2 Selectivity. Frontiers in Pharmacology, 7, 503.
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8

Differentiation of C2C12 Myoblasts

Cited 1 time
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C2C12 myoblasts were obtained from the American Type Culture Collection (Rockville, MD, USA). C2C12 cells were cultured in Dulbecco’s Minimum Essential Medium (DMEM) containing 10% FetalPlex (Gemini Bio-Products, Woodland, CA) and 1% penicillin/streptomycin. C2C12 cells were passaged by using a 5% trypsin, 0.02% EDTA solution every one to two days. Cells were differentiated for two days by incubating with DMEM containing 2% horse serum and 1% penicillin/streptomycin. Cells were grown in a humidified incubator with 5% CO2 at 37 °C. For some experiments, myotubes were serum-starved by incubation with DMEM with antibiotic but without FetalPlex for three hours. C2C12 cell lines expressing green fluorescent protein (GFP) or short hairpin RNA to reduce ATM expression were described previously [5 , 18 (link)].
Spears L.D., Tran A.V., Qin C.Y., Hobbs S.B., Liang Burns C.A., Royer N.K., Zhang Z., Ralston L, & Fisher J.S. (2016). Chloroquine increases phosphorylation of AMPK and Akt in myotubes. Heliyon, 2(3), e00083.
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9

Culture of L6 Myoblasts

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L6 myoblasts were bought from the American Type Culture Collection (Manassas, VA). DMEM containing 10% FetalPlex (Gemini Bio-Products, Woodland, CA) and 1% antibiotic•antimycotic solution were used to culture the L6 myoblasts at 37 °C in 5% CO2.
Andrisse S., Koehler R.M., Chen J.E., Patel G.D., Vallurupalli V.R., Ratliff B.A., Warren D.E, & Fisher J.S. (2014). Role of GLUT1 in regulation of reactive oxygen species. Redox Biology, 2, 764-771.
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10

GPC3 Expression in Carcinoma Cell Lines

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Cell lines include a GPC3 -human epidermoid carcinoma (A431), transfected A431-GPC3 + (G1) (31) , as well as GPC3 + human hepatoblastoma (HepG2), human hepatocellular carcinoma (Hep3B), which were obtained from ATCC (Manassas, VA). A well-differentiated human hepatocellular carcinoma, Huh7 (GPC3 low ), was obtained from Sekisui Xenotech (Kansas City, KS). DMEM (Life Technologies, Carlsbad, CA) supplemented with 10% FetalPlex (Gemini Bio-Products, West Sacramento, CA) was used to culture all cell lines. All cell lines tested negative for mycoplasma in monthly tests and were used for experiments within 15 passages.
Kelada O.J., Gutsche N.T., Bell M.M., Berman R., Baidoo K.E., Warner B.M., Szajek L.P., Hong J., Ho M., Choyke P.L., & Escorcia F.E. (2020). ImmunoPET as Stoichiometric Sensor for Glypican-3 in Models of Hepatocellular Carcinoma.
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