Polydimethylsiloxane divinylbenzene pdms dvb

The Chinese rose cultivars have different colors and fragrances, and the four cultivars selected in this study had large color differences. Four cultivars (Rosa ‘Funkuhr’, R. ‘Pink Panther’, R. ‘Crimson Glory’, and R. ‘Blue River’, denoted RF, RPP, RCG and RBR, respectively) were generously provided by the experimental field of Guizhou Botanical Garden and harvested at E 106° 40’–27”; N 26° 11’ 39”, located in Guiyang City, China (Figure 1). The flowers were harvested before petal opening and transferred to the laboratory. The samples were powdered following the method described in literatures (Qian et al., 2019 (link); Gil et al., 2020 (link)). Methanol was purchased from Sigma−Aldrich Fluka (Buchs, Switzerland). Methyl hexadecanoate (internal standard; purity ≥ 97.5%) was obtained from CATO (Portland, OR, USA) and stored at 4°C until use. Commercially available SPME coating fibers, including 85 µm carboxen/polydimethylsiloxane (CAR/PDMS), 50/30 µm divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS), 65 µm polydimethylsiloxane/divinylbenzene (PDMS/DVB), and 60 µm carbowax-polyethylene glycol (PEG), were purchased from Supelco (Bellefonte, PA, USA).

Yangchun is located in southwest of Guangdong province of China (111° 16′ 27″ to 112° 09′ 22″ E, 21° 50′ 36″ to 22° 41′ 01″ N), which is the geo-authentic habitats of A. villosum. Seventy-two batches of fresh A. villosum samples containing fruits (1–18), roots (19–36), leaves (37–54) and stems (55–72) were obtained from different places of Yangchun. The detailed characteristics are presented in Table 1. Species identification was performed by Dr. Jing Zhao and Dr. Hao Hu. The voucher specimens of these samples were deposited at the Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China.Table 1

Characteristics of analyzed samples

Codes	Sample	Source
1–9	Fruits	Pingxi village, Yangchun
10–18	Fruits	Shiwanzai village, Yangchun
19–27	Roots	Pingxi village, Yangchun
28–36	Roots	Shiwanzai village, Yangchun
37–45	Leaves	Pingxi village, Yangchun
46–54	Leaves	Shiwanzai village, Yangchun
55–63	Stems	Pingxi village, Yangchun
64–72	stems	Shiwanzai village, Yangchun

All other chemicals and reagents were of analytical grade. The extraction fibers: 100 μm polydimethylsiloxane (PDMS), 65 μm polydimethylsiloxane/divinylbenzene (PDMS/DVB), 85 μm polyacrylate and 75 μm carboxen poly(dimethylsiloxane) (CAR/PDMS) were purchased from Supelco (Bellefonte, PA, USA). Headspace vials (20 mL) and accessories were obtained from Agilent (Palo Alto, CA, USA).

A total of 11 batches of Zhejiang SD (batch number ZJ01, ZJ02, ZJ03, ZJ04, ZJ05, ZJ06, ZJ07, ZJ08, ZJ09, ZJ10, and ZJ11) and 13 batches of Guizhou SD (batch number GZ01, GZ02, GZ03, GZ04, GZ05, GZ06, GZ07, GZ08, GZ09, GZ10, GZ11, GZ12, and GZ13) were purchased from Hangzhou Chinese Herbal Medicine Trading Center (Zhejiang Province) and Guiyang Chinese Herbal Medicine Market (Guizhou Province). These samples were authenticated by Zhang Yujin (an associate professor from Zunyi Medical University) and deposited in the chemical laboratory. n-Hexane of analytical grade was purchased from Suzhou Boyuan Chemical Co., LTD (Suzhou, China). n-Alkane (C₈–C₃₀) series were provided by Sa Lan Chemical Co., LTD (Shanghai, China). SPME holder and 100 µm Polydimethylsiloxane (PDMS), 75 µm Carboxen/Polydimethylsiloxane (CAR/PDMS), 65 µm Polydimethylsiloxane/Divinylbenzene PDMS/DVB, and 50/30 µm Divinylbenzene/Carboxen/ Polydimethylsiloxane (DVB/CAR/PDMS) fiber were purchased from Supelco Inc (Bellefonte, PA, USA). HS-SPME unit and transparent sample bottle were purchased from Kanglin Technology Co., LTD (Beijing, China).