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A549 human nsclc cells

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The A549 human non-small cell lung cancer (NSCLC) cells are a well-characterized in vitro model system derived from a human lung adenocarcinoma. These cells can be used for various research applications, including the study of lung cancer biology, drug screening, and toxicology assays.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Maixin Group (1 mentions) Penicillin streptomycin, by American Type Culture Collection (1 mentions) 100 mm plastic cell culture dishes, by BD (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Sclc calu 6 cells, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin liquid, by Solarbio (1 mentions) Nih 3t3 mouse embryo fibroblasts, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Cytiva (1 mentions) L glutamine, by Cytiva (1 mentions) Solubilization stop solution, by Promega (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Promega (1 mentions) Mda mb 231 breast cancer cells, by American Type Culture Collection (1 mentions) Mcf 10a normal breast epithelial cells, by American Type Culture Collection (1 mentions)

7 protocols using a549 human nsclc cells

1

NSCLC A549 Cell Culture Protocol

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Human NSCLC A549 cells (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% (v/v) fetal bovine serum (FBS) and penicillin–streptomycin (100 U/mL; 100 µg/mL) at 37°C in a 5% CO2 humidified incubator. DMEM, FBS, and penicillin–streptomycin were purchased from Maixin Bio (Fuzhou, China).
Chen Y., Xu Y., Zhu K., Cao Z, & Huang Z. (2019). Tumor necrosis factor-related apoptosis-inducing ligand modulates angiogenesis and apoptosis to inhibit non-small cell lung carcinoma tumor growth in mice. The Journal of International Medical Research, 47(7), 3243-3252.
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2

Culturing Human Lung Cell Lines

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Human NSCLC A549 cells and SCLC Calu-6 cells were obtained from the American Type Culture Collection (Manassas, VA, USA). Primary normal HPF cells were purchased from PromoCell GmbH (C-12360, Heidelberg, Germany) and used between passages five and seven. These lung cells were kept in a standard humidified incubator at 37 °C with 5% CO2 and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (Sigma-Aldrich Co., St. Louis, MO, USA) and 1% penicillin-streptomycin (Gibco BRL, Grand Island, NY, USA). Cells were grown in 100 mm plastic cell culture dishes (BD Falcon, Franklin Lakes, NJ, USA) and harvested with trypsin-EDTA (Gibco BRL).
, & Park W.H. (2023). Ebselen Inhibits the Growth of Lung Cancer Cells via Cell Cycle Arrest and Cell Death Accompanied by Glutathione Depletion. Molecules, 28(18), 6472.
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3

Culturing NSCLC and Normal Bronchial Cells

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A549 human NSCLC cells and BEAS-2B human normal bronchial epithelial cells were acquired from American Type Culture Collection (ATCC, Manassas, VA). Both cells were cultured in RPMI 1640 medium (11875093, Thermo Fisher Scientific) supplemented with 10% v/v exosome-depleted fetal bovine serum (FBS, 26140079, Thermo Fisher Scientific) and 1× penicillin-streptomycin (PS, 15140122, Thermo Fisher Scientific). To prepare exosome-depleted FBS, the FBS was centrifuged at 100,000g at 4 °C for 3 h to remove the exosomes and then sterilized by filtration through 0.22 μm filters. The A549 and BEAS-2B cells were subcultured every 2–3 days.
Yang Y., Kannisto E., Yu G., Reid M.E., Patnaik S.K, & Wu Y. (2018). An Immuno-Biochip Selectively Captures Tumor-Derived Exosomes and Detects Exosomal RNAs for Cancer Diagnosis. ACS applied materials & interfaces, 10(50), 43375-43386.
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4

Culturing Cancer and Fibroblast Cell Lines

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A549 human NSCLC cells, NIH3T3 mouse embryo fibroblasts, and IMR90 human lung embryo fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA). A549 and IMR90 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin liquid (Solarbio Life Sciences, Beijing, China). The cells were maintained at 37°C in a humidified incubator with 5% CO2.
Sun H.N., Guo X.Y., Xie D.P., Wang X.M., Ren C.X., Han Y.H., Yu N.N., Huang Y.L, & Kwon T. (2022). Knockdown of Peroxiredoxin V increased the cytotoxicity of non-thermal plasma-treated culture medium to A549 cells. Aging (Albany NY), 14(9), 4000-4013.
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5

Culturing A549 NSCLC Cells

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A549 human NSCLC cells were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). The cells were cultured in RPMI medium (Wellgene Laboratories, Daegu, Korea) supplemented with 2 mM L-glutamine and 10% fetal bovine serum (Hyclone Laboratories, Rockford, IL, USA) and incubated under humidified conditions at 37 °C with 5% CO2. The PTX-resistant A549 cell line (A549/PTX) was prepared and gifted by Dr Lee.24 (link)
Ham S.Y., Kwon T., Bak Y., Yu J.H., Hong J., Lee S.K., Yu D.Y, & Yoon D.Y. (2016). Mucin 1-mediated chemo-resistance in lung cancer cells. Oncogenesis, 5(1), e185-.
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6

Evaluation of NSCLC Cell Viability

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A549 human NSCLC cells (American Type Culture Collection, Manassas, VA, USA) were cultured in DMEM-S to 80% confluence and then in serum-free DMEM for 24 h at 37°C in a 5% CO 2 atmosphere. Next, the tumor cells were cultured in 96-well plates (5,000 cells per well) in the presence of CM from CAFs or NFs for 72 h at 37°C in a 5% CO 2 atmosphere. Then, 15 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Promega Corporation, Madison, WI, USA) was added to each well, and the plate was incubated for 4 h at 37°C. Finally, 100 μL of solubilization/ stop solution (Promega) was added, and the absorbance was measured at 570 nm. Tumor cells were also cultured in serum-free DMEM as controls.
Negrete-Garcia M.C., Ramírez-Rodriguez S.L., Rangel-Escareño C., Muñoz-Montero S., Kelly-García J., Vázquez-Manríquez M.E., Santillán P., Ramírez M.M., Ramírez-Martínez G., Ramírez-Venegas A, & Ortiz-Quintero B. (2018). Deregulated MicroRNAs in Cancer-Associated Fibroblasts from Front Tumor Tissues of Lung Adenocarcinoma as Potential Predictors of Tumor Promotion. The Tohoku journal of experimental medicine, 246(2).
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7

Comparative Cell Culture Protocols

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A549 human NSCLC cells, BEAS-2B human normal bronchial epithelial cells, MDA-MB-231 breast cancer cells and MCF-10A normal breast epithelial cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, United States). A549 and BEAS-2B cells were cultured in RPMI 1640 medium containing 10% v/v FBS and 1× PS. A549 and BEAS-2B cells were seeded at 1.0 × 106 and 1.5 × 106 cells/P100 dish, respectively, and subcultured every 2–3 days. MDA-MB-231 cells were cultured in DMEM medium containing 10% v/v FBS and 1× PS. MDA-MB231 cells were seeded at 1.5 × 106 cells/P100 dish and subcultured every 2–3 days. MCF-10A cells were cultured in DMEM/F-12 medium containing 10% v/v FBS and 1× PS. MCF-10A cells were seeded at 2 × 106 cells/P100 dish and subcultured every 2–3 days.
Hsu C.C., Yang Y., Kannisto E., Zeng X., Yu G., Patnaik S.K., Dy G.K., Reid M.E., Gan Q, & Wu Y. (2023). Simultaneous Detection of Tumor Derived Exosomal Protein–MicroRNA Pairs with an Exo-PROS Biosensor for Cancer Diagnosis. ACS nano, 17(9), 8108-8122.
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