Colletotrichum acutatum

Endophytic Streptomyces sp. SP5 (GenBank accession number MW564023) isolated from Citrus jambhiri leaves was used in the current study^{14 (link)}. The streptomycete was maintained on starch casein nitrate agar (SCNA) plates, and spores of isolate were preserved in 20% glycerol at − 20 °C as stock for future use. Different test pathogenic bacteria viz. Bacillus subtilis (MTCC 619), Klebsiella pneumoniae subsp. pneumoniae (MTCC 109), E. coli (MTCC 1885), Staphylococcus epidermidis (MTCC 435), Salmonella typhi (MTCC 733), Alternaria brassicicola (MTCC2102), Colletotrichum acutatum (MTCC1037), Alternaria solani (MTCC2101), Cladosporium herbarum (MTCC351) and Fusarium oxysporum (MTCC284) were obtained from the Microbial Type Culture Collection (MTCC) and Gene Bank, CSIR-Institute of Microbial Technology (IMTECH), Chandigarh, India. Fusarium solani (NFCCI 91) was obtained from the NFCCI (National Fungal Culture Collection of India), Pune, and Alternaria alternata (accession number GU004283) and Fusarium moniliforme were isolated in the lab (PAU). VRE (resistant to methicillin, vancomycin, imipenem, and clindamycin) and MRSA (resistant to methicillin, teicoplanin, imipenem, and clindamycin) were collected from local hospitals. The bacterial and fungal cultures were maintained at 4 °C on nutrient agar and potato dextrose agar slants, respectively.

Different test phytopathogenic fungi viz. Alternaria brassicicola (MTCC2102), Alternaria solani (MTCC2101), Colletotrichum acutatum (MTCC1037), Fusarium oxysporum (MTCC284), Cladosporium herbarum (MTCC351) were procured from Microbial Type Culture Collection (MTCC), CSIR-Institute of Microbial Technology (IMTECH), Chandigarh, India. Whereas, Fusarium solani (NFCCI 91) was obtained from the National Fungal Culture Collection of India NFCCI, Pune. Fusarium sp. and Alternaria sp. (accession number GU004283) were isolated in the lab [49 ]. All the fungal cultures were maintained at 4 °C on Potato dextrose agar (PDA) slants.

Streptomyces hydrogenans strain DH16 (GenBank accession no. JX123130) was isolated from soil procured from Dalhousie (32.53° N, 75.98° E), Himachal Pradesh, India (Kaur and Manhas, 2014 (link)) and maintained on starch casein nitrate agar slants at refrigeration temperature (4°C). Twenty percent glycerol stocks were also prepared and stored at -20°C during the study. The three test phytopathogenic fungi viz. Alternaria brassicicola (MTCC 2102), A. solani (MTCC 2101), and Colletotrichum acutatum (MTCC 1037) were obtained from Microbial Type Culture Collection (MTCC), Institute of Microbial Technology (IMTECH), Chandigarh, India. Fusarium moniliforme, Alternaria alternata were isolated in lab. All the fungal cultures were maintained on Potato dextrose agar (PDA) slants at 4°C.