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2 protocols using n propanol

1

Synthesis and Characterization of BP(OH)2

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[2,2′-Bipyridyl]-3,3′-diol,
(BP(OH)2) was purchased from Sigma-Aldrich and used as
received without further purification. Spectroscopy-grade DMSO, ethanol, n-propanol, and tert-butyl alcohol (TBA)
were obtained from Spectrochem Pvt. Ltd., India, and distilled before
use. Triple-distilled Milli-Q water was used to prepare all of the
solutions of TBA–water, ethanol–water, n-propanol–water, and DMSO–water binary mixtures.
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2

Purification and Characterization of L-Asparaginase

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Culture media components such as tryptone extract and Bacto yeast extract were purchased from Difco Laboratories, India. Phenylmethylsulfonyl fluoride (PMSF), isopropyl β-D-1-thiogalactopyranoside (IPTG), acrylamide, bis-acrylamide, Tris–HCl, and L-asparagine were from Amresco, United States. Sodium dodecyl sulfate (SDS), ammonium persulfate (APS), dithiothreitol (DTT), urea, Nessler’s reagent, and trichloroacetic acid (TCA) were from Sigma-Aldrich, United States. 2,2,2-Trifluoroethanol was from SRL, India. Tetramethyl ethylenediamine (TEMED), ethylenediaminetetraacetic acid (EDTA), and bromophenol blue were from Bio-Rad, United States. Coomassie Brilliant Blue R-250 and ampicillin were from USB Corporation, United States. Glacial acetic acid and methanol were from Merck’s EMPARTA®. Ethanol, n-propanol, and glycerol were of analytical grade and were from Spectrochem, India. DEAE-Sepharose Fast Flow media was purchased from GE Healthcare, United Kingdom. BCA assay kit, SDS-PAGE prestained molecular weight marker, and commercial r-hGH were from Thermo Fisher Scientific, United States. Commercial L-asparaginase 5,000 IU/ml, Bionase® 5K, was purchased from Zydus Cadila, India. RPMI, horse serum (HS), and fetal bovine serum (FBS) were from Gibco BRL, United States. All the other chemicals were of analytical grade.
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