Culture media components such as tryptone extract and
Bacto yeast extract were purchased from Difco Laboratories, India.
Phenylmethylsulfonyl fluoride (PMSF), isopropyl β-
D-1-thiogalactopyranoside (IPTG), acrylamide,
bis-acrylamide,
Tris–HCl, and
L-asparagine were from Amresco, United States. Sodium dodecyl sulfate (SDS), ammonium persulfate (APS), dithiothreitol (DTT), urea,
Nessler’s reagent, and
trichloroacetic acid (TCA) were from Sigma-Aldrich, United States. 2,2,2-Trifluoroethanol was from SRL, India. Tetramethyl ethylenediamine (TEMED), ethylenediaminetetraacetic acid (EDTA), and
bromophenol blue were from Bio-Rad, United States.
Coomassie Brilliant Blue R-250 and
ampicillin were from USB Corporation, United States. Glacial acetic acid and methanol were from Merck’s EMPARTA
®. Ethanol,
n-propanol, and glycerol were of analytical grade and were from Spectrochem, India.
DEAE-Sepharose Fast Flow media was purchased from GE Healthcare, United Kingdom.
BCA assay kit, SDS-PAGE prestained molecular weight marker, and commercial r-hGH were from Thermo Fisher Scientific, United States. Commercial
L-asparaginase 5,000 IU/ml, Bionase
® 5K, was purchased from Zydus Cadila, India.
RPMI,
horse serum (HS), and
fetal bovine serum (FBS) were from Gibco BRL, United States. All the other chemicals were of analytical grade.
Singhvi P., Verma J., Panwar N., Wani T.Q., Singh A., Qudratullah M., Chakraborty A., Saneja A., Sarkar D.P, & Panda A.K. (2021). Molecular Attributes Associated With Refolding of Inclusion Body Proteins Using the Freeze–Thaw Method. Frontiers in Microbiology, 12, 618559.