Hla dr bv421
HLA-DR-BV421 is a fluorochrome-conjugated antibody that binds to the HLA-DR antigen, a major histocompatibility complex (MHC) class II cell surface receptor. It is used in flow cytometry applications to identify and quantify HLA-DR-expressing cells.
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7 protocols using hla dr bv421
Phenotyping and Proliferation of Anti-SHIV T Cells
Imaging Flow Cytometry Characterization of EVs
Comprehensive Immune Cell Profiling of HPSCC
Single-cell suspensions were resuspended in FACS buffer (PBS + 2% FBS) and incubated with anti-CD16/CD32 (BioLegend, 156603) for 20 min on ice. For cell membrane staining, the samples were first stained with Dead Cell Stain (Thermo Fisher, USA) for 15 min at 25 ℃ and then incubated with the cell surface markers CD4 (FITC), CD127 (APC), CD11b (PerCP/Cy5.5) from eBioscience, CD45 (Spark BlurTM550), CD3 (AF700), CD25 (PE/Cy7), PD-1 (PE/Dazzle594), CCR7 (Spark NIRTM685), CD11C (BV510), HLA-DR (BV421), and PD-L1(BV650) from Biolegend, and LAMP3 (PE, BD) for 30 min at 4 °C. Spectroscopic flow cytometry was performed for detection and analysis.
Multicolor Flow Cytometry of MSCs
Flow cytometry analysis of PBMCs
Flow Cytometry Analysis of PBMCs
Isolation and Characterization of Distinct CD4+ T Cell Subsets
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