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Solarix xr instrument

Manufactured by Bruker
Sourced in Germany

The SolariX XR instrument is a high-performance Fourier Transform Ion Cyclotron Resonance (FT-ICR) mass spectrometer designed for advanced analytical applications. It offers high mass resolving power, mass accuracy, and sensitivity, enabling precise analysis of complex samples.

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3 protocols using solarix xr instrument

1

MALDI-TOF MS Peptide Characterization

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The MTP 384 polished steel target plate containing hit substrate
samples from library screening was read on a Bruker solariX XR instrument.
An initial MALDI spectrum was obtained in the 150–2500 m/z range using the following parameters:
a laser frequency = 200 Hz and collision energy = 14.0 V combined
with a raster smart walk pattern. This provided the mass of the quasi-molecular
ion; namely the singly protonated intact peptide [M + H]+. Using collision-induced dissociation (CID), the quasi-molecular
ion was fragmented with a laser frequency = 200 Hz, collision cell
radiofrequency (RF) = 1.6 Vpp and collision energy = 80.0 V using
a raster smart walk pattern. This generated a tandem mass spectrum
also in the 150–2500 m/z range.
The data for each hit peptide was collected and analyzed using Bruker
Compass DataAnalysis 5.1.
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2

Spectroscopic Characterization of Surfactants

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The characterization of intermediate (4) and gemini surfactants (GS1–3) were done with the aid of FT-IR, 1H-NMR, 13C-NMR, and MALDI MS techniques. The FT-IR data was obtained using a 16F PerkinElmer FT-IR spectrometer. The NMR analysis was conducted on Jeol 1500 spectrometer and the samples were dissolved in chloroform-d tetramethylsilane as an internal standard. The MALDI mass spectrum was obtained from Bruker SolariX XR instrument in a matrix of dithranol in dichloromethane.
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3

Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry

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Fourier-transform ion cyclotron
resonance mass spectra were acquired in a positive ion mode on a SolariX
XR instrument with a 9.4 T actively shielded superconducting magnet
and a dynamically harmonized cell (Bruker Daltonics, Bremen, Germany)
fitted with a nano-electrospray online source. Samples (1 mg/mL in
acetone) were introduced in the MS using a syringe pump (Cole-Parmer,
USA) at a rate of 10 μL/min, connected to a capillary line (100
μm i.d., 360 μm o.d.), equipped with a SilicaTip needle
(10 ± 1 μm, PicoTip Emitter, New Objective, USA), to which
a potential of 1.3 kV was applied. The front and back trapping potentials
were set at 1.6 V with a skimmer voltage at 10 V, and the ions were
accumulated in the hexapole for 0.01 s. Spectra were acquired using
broadband detection, 16 M data points, 150 scans accumulation, and
a mass range of m/z 144 to 2000.
The instrument was calibrated using sodium trifluoroacetate (0.01
mg/mL in H2O/MeOH 50/50 v/v) with a linear calibration.
Spectra were internally calibrated in the Data Analysis software v.5.0
(Bruker Daltonics, Germany) using a list of assigned signals.
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