Expression of stemness-associated marker, CD24 and CD44, was detected by flow cytometer. Cells were plated in 6-well plates and transfected with siRNA for 10 h. After continuous culture of 48 h, cells were digested with trypsin-EDTA and washed twice in ice-cold PBS containing 2% BSA and centrifuged at 300 ×g for 3 min. Cells were divided into two groups and resuspended in 100 μl PBS with 2% BSA on ice. Then the antibody APC-IgG, PE-IgG and APC-CD44, PE-CD44 (BD Pharmingen) were respectively added into single tube of each group on ice to incubate for 30 min. The fluorescence value was detected finally by flow cytometer.
Pe igg
Manufactured by BD
PE-IgG is a laboratory reagent used for the detection and analysis of immunoglobulin G (IgG) molecules. It is a fluorescent-labeled antibody that binds specifically to IgG proteins, allowing for their identification and quantification in various sample types.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pe igg
1
Quantification of Stemness Markers
2
Flow Cytometric Analysis of CD66 and CD18
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse anti‐human CD66‐PE (Catalog #: 551480; recognizing CD66a, CD66c, CD66d, and CD66e subunits), PE IgG (Catalog #: 555787), mouse anti‐human CD18‐FITC (Catalog #: 557156), and FITC IgG (Catalog #: 555786) were from BD Biosciences. Following manufacturer's recommendations, the dilutions of antibodies and control IgG for experiments were 1:5. Flow cytometric analysis was performed as previously described (Ding et al, 2013 ). Data were acquired and analyzed with FlowJo software (version 7.6.5; Tree star, Ashland, OR).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!