Taqman pcr reagents

Manufactured by Thermo Fisher Scientific

Taqman PCR reagents are a set of chemical compounds used in the process of Polymerase Chain Reaction (PCR) to detect and quantify specific DNA sequences. These reagents include enzymes, buffers, and fluorescent probes that enable the amplification and detection of target DNA molecules during the PCR thermal cycling process.

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Lab products found in correlation

Abi prism 7700, by Thermo Fisher Scientific (1 mentions) Superscript 2 rt kit, by Thermo Fisher Scientific (1 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (1 mentions) Nanodrop, by Agilent Technologies (1 mentions)

Close spelling variants of this product

TaqMan universal PCR master mix reagents kit Taqman qRT-PCR reagents TaqMan Universal Fast PCR master mix reagents TaqMan® Reverse Transcription and Universal PCR Master Mix Reagents Taqman one-step RT-PCR reagents TaqMan EZ RT-PCR core reagents kit TaqMan PCR master mix reagents TaqMan PCR core reagents TaqMan RT-PCR reagents TaqMan EZ RT-PCR Core Reagents

2 protocols using taqman pcr reagents

Real-Time RT-PCR for GSC Expression

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Real-time reverse transcription-PCR (RT-PCR) was established using Taqman PCR reagents and ABI PRISM 7700 sequence detection system (Applied Biosystems, Foster, CA) in accordance with the protocol described previously [18] (link). The primers used for GSC amplification were described previously [16] (link). The assay was performed in triplicate, and the results were analyzed using Student's t test.

Xue T.C., Ge N.L., Zhang L., Cui J.F., Chen R.X., You Y., Ye S.L, & Ren Z.G. (2014). Goosecoid Promotes the Metastasis of Hepatocellular Carcinoma by Modulating the Epithelial-Mesenchymal Transition. PLoS ONE, 9(10), e109695.

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Quantifying miR-29b Expression

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As described previously [30 ], total RNA was isolated using a Trizol-based (Invitrogen) RNA isolation protocol. RNA was quantified by Nanodrop (Agilent Technologies), and RNA and miRNA quality were verified using an Agilent 2100 Bioanalyzer (Agilent Technologies). First strand cDNA synthesis was performed for each RNA sample using the Superscript II RT Kit (Applied Biosystems, New York, USA). Real time quantitative polymerase chain reaction was performed using Taqman PCR reagents (Applied Biosystems), and the primers for miR-29b were designed according to the manufacturer's recommendations. The relative amount of miRNA was standardized against U6 snRNA, and the fold change for miR-29b was calculated using the 2-DD computed tomography method.

Wang F., Ma H., Liang W.J., Yang J.J., Wang X.Q., Shan M.R., Chen Y., Jia M., Yin Y.L., Sun X.Y., Zhang J.N., Peng Q.S., Chen Y.G., Liu L.Y., Li P., Guo T, & Wang S.X. (2017). Lovastatin upregulates microRNA-29b to reduce oxidative stress in rats with multiple cardiovascular risk factors. Oncotarget, 8(6), 9021-9034.

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