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Axio observer

Manufactured by Merck Group
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Sourced in Denmark

The Axio Observer is a high-performance inverted microscope designed for a wide range of applications. It features a robust and stable construction, providing reliable performance and precise imaging. The microscope is equipped with advanced optics, ensuring clear and detailed observations.

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Lab products found in correlation

Stempro chondrogenic differentiation kit, by Thermo Fisher Scientific (1 mentions) Alcian blue 8gx, by Merck Group (1 mentions) Fm4 64, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Axio Observer.Z1

2 protocols using axio observer

1

Chondrogenic Differentiation of ASCs

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ASCs were seeded in a 96-well V-shaped tissue culture plate (Greiner Bio-one, Frickenhausen, Germany) at 80,000 cells per well, centrifuged at 500× g for 5 min, and incubated for 3 weeks with the STEMPRO® Chondrogenic-differentiation kit (ThermoFisher Scientific, Life Technologies, Roskilde, Denmark). The control pellets were maintained in a growth medium. Hereafter, the pellets were embedded in 5 µm paraffin sections, the sulfated glycosaminoglycans (GAGs) stained with Alcian Blue 8GX (Sigma-Aldrich, Søborg, Denmark) for 30 min, and the degree of differentiation was evaluated by bright field microscopy (Axio Observer).
Ren G., Peng Q., Emmersen J., Zachar V., Fink T, & Porsborg S.R. (2022). A Comparative Analysis of the Wound Healing-Related Heterogeneity of Adipose-Derived Stem Cells Donors. Pharmaceutics, 14(10), 2126.
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2

GzmB and GNLY-mediated bacterial lysis

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GzmB (100 nM) and sublytic GNLY (20 nM for this strain) were added at 37°C in hypotonic buffer (one third deionized water, two thirds HBSS) to 107 exponential phase Ec ectopically expressing GFP-ssrA for indicated times. After washing for 10 min in HBSS, bacteria were fixed with 2% formalin and stained with 4′,6-diamidino-2-phenylindole (DAPI) and FM4-64 (Molecular Probes) for 30 min on ice. Stained bacteria were mounted on glass slides using VINOL mounting medium (Sigma) for fluorescence microscopy using an inverted, fully motorized Axio Observer spinning disk microscope. Images were analyzed with SlideBook V5.0 software. Bacterial cell masks were assigned based on FM4-64 fluorescence, and GFP-ssrA fluorescence within the mask for each bacterium was measured to obtain mean fluorescence intensity/cell in at least 15 fields/slide.
Dotiwala F., Santara S.S., Binker-Cosen A.A., Li B., Chandrasekaran S, & Lieberman J. (2017). Granzyme B disrupts central metabolism and protein synthesis in bacteria to promote an immune cell death program. Cell, 171(5), 1125-1137.e11.
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