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Alt detection kit

Manufactured by Nanjing Jiancheng
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The ALT detection kit is a laboratory tool used to measure the levels of the enzyme alanine aminotransferase (ALT) in biological samples. ALT is an enzyme found primarily in the liver and is often used as a biomarker to assess liver function. The kit provides the necessary reagents and protocols to accurately quantify ALT concentrations.

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Lab products found in correlation

Ast detection kit, by Nanjing Jiancheng (2 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Varioskan flash, by Thermo Fisher Scientific (1 mentions) D galn, by Merck Group (1 mentions)

Close spelling variants of this product

ALT Kit (9 citations) ALT and AST detection kits (4 citations) Detection kits of ALT (2 citations)

2 protocols using alt detection kit

1

Liver Function Enzyme Assay

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An ALT detection kit (Nanjing Jiancheng Bioengineering Institute, cat. no. C009-2, batch no. 20141105) and an AST detection kit (Nanjing Jiancheng Bioengineering Institute, cat. no. C0010-2, batch no. 20141104) were used in strict accordance with the manufacturer's protocol.
Ding H, & Wen Z. (2018). Overexpression of C-sis inhibits H2O2-induced Buffalo rat liver cell apoptosis in vitro and alleviates liver injury in a rat model of fulminant hepatic failure. International Journal of Molecular Medicine, 42(2), 873-882.
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2

Murine Model of Acute Liver Failure

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We established the mice model of ALF by intraperitoneally injecting LPS (5 mg/kg body weight; Sigma-Aldrich) and D-GalN (100 mg/kg body weight; Sigma-Aldrich) into SND1 LKO or WT mice. Normal saline (NS) was used as a control. Serum aminotransferase activities of mice were measured using an aspartate aminotransferase (AST) detection kit (Jiancheng, Nanjing), an alanine aminotransferase (ALT) detection kit (Jiancheng, Nanjing), and a microplate reader (Varioskan Flash, Thermo). We also perform an H&E staining assay in different groups using Hematoxylin and eosin (ZSGB-BIO), according to the manufacturer’s instructions.
Zhao C., Cui X., Zhao Y., Qian B., Zhang N., Xin L., Ha C., Yang J., Wang X, & Gao X. (2021). Impact of hepatocyte-specific deletion of staphylococcal nuclease and tudor domain containing 1 (SND1) on liver insulin resistance and acute liver failure of mice. Bioengineered, 12(1), 7360-7375.
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