Typical procedure for bicompartmental microfibers is as follows. Two different polymer solutions were prepared in separate vials. 30 w/v% of PLGA was dissolved in a solvent mixture of chloroform and DMF (95 : 5, v/v). Another solution contained 30 w/v% of PLGA and COT-PLA with a concentration of 9 w/v % (30 % by weight of PLGA), which were dissolved in the same ratios of solvents. More detailed information for the preparation of microfibers created in this work is stated in Table S1 . Experimental setup includes a syringe pump (Fisher Scientific, Inc., USA), a power supply (DC voltage source, Gamma High Voltage Research, USA), and a rotary collector. Each set of two polymer solutions was delivered at a constant flow rate of 0.05 ml/h via vertically positioned syringes equipped with 26 G needles (Hamilton Company, USA). When a driving voltage of 11~12 kV was applied to the polymer solution, stable Taylor Cone was formed and fibers were collected at a distance of 7 cm.
Syringe pump
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Syringe Pump is a precision instrument used to accurately deliver controlled volumes of liquid samples or reagents. It utilizes a stepper motor to drive a syringe plunger, enabling precise and repeatable dispensing of liquids. The Syringe Pump is designed for a wide range of applications in laboratory settings, providing consistent and reliable fluid delivery.
Automatically generated - may contain errors
Lab products found in correlation
Hexane, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Levofloxacin, by Merck Group (1 mentions)
Pluronic f127, by BASF (1 mentions)
Ils 300lm, by Newport Corporation (1 mentions)
Xps q4, by Newport Corporation (1 mentions)
C18 ziptip, by Merck Group (1 mentions)
Di water, by Thermo Fisher Scientific (1 mentions)
26 g needle, by Terumo (1 mentions)
Fluorescent beads, by Thermo Fisher Scientific (1 mentions)
14 protocols using syringe pump
1
Bicompartmental Microfiber Fabrication
2
Electrospinning for Coagulation Studies
3
Levofloxacin-Loaded PLLA Microfiber Sutures
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Levofloxacin microfiber sutures were manufactured using the wet electrospinning setup depicted in Figure 1A and described by Zhang and coworkers.37 (link) Briefly, PLLA (221 kDa; Corbion, Amsterdam, Netherlands) at 86% to 89% (wt/wt) was mixed with Levofloxacin (Sigma Aldrich, St. Louis, MO) at 10 wt% and either PEG (35 kDa; Sigma Aldrich) or Pluronic F127 (BASF, Florham Park, NJ) between 1 and 4 wt% and dissolved in chloroform (Sigma Aldrich) at room temperature for 24 hours. Levofloxacin concentration was held constant and PLLA concentration in chloroform was maintained at 15 wt% in all formulations. Sutures were produced by wet electrospinning the polymer/drug solution in a setup consisting of a high voltage power supply (Gamma High Voltage Research, Ormond Beach, FL), syringe pump (Fisher Scientific, Waltham, MA), and rotating metal collector with hexane (Sigma Aldrich) as the lending solvent. The polymer solution was ejected through a blunted 18-G needle (Fisher Scientific) at 13 mL/h with 4.7 kV of applied voltage 5 cm away from the collector rotating at 40 rpm. Fibers were then collected and desiccated for 2 days prior to storage at −20°C.
4
Electrochemical detection of Trp and Tyr
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A continuous flow of PBS (buffer) was pumped into a flow cell (built in-house, Cole-Parmer, Vernon Hills, IL) at 1.81 mL min−1 using a syringe pump (Fisher Scientific, Waltham, MA, USA). Reference and working electrodes were attached to the flow cell accordingly. A 5 s bolus of Trp, Tyr, or both was introduced to the electrodes by using a 6-port injection valve (VICI, Houston, TX, USA).
5
Electrospun Alginate Bead Formation
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The alginate solution was extruded using a syringe pump (Fisher Scientific, Illkirch, France) through a needle connected to an electrostatic energy generator (Bertan, USA, positive connector). This generator was placed above a beaker containing the calcium solution and connected to the negative but grounded connector of the electrostatic generator. The needle was purchased from Eleco Produits (Gennevilliers, France) and fixed on a 3 D printed build block through luer lock connectors (Fisher Scientific, Illkirch, France) (Figure 1).
6
Bicompartmental Microfiber Fabrication
7
Quantitative Mass Spectrometry Analysis
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A Q Exactive plus (Thermo Fisher Scientific) was used to carry out electrospray ionization-mass spectrometry experiments. The mass spectrometer was operated at resolution 280,000, spray voltage 3.5 kV, and capillary temperature 320°C. Samples were desalted by C18 Zip-tip (Millipore) and dissolved in 100 μl of reconstitution buffer (50% ACN, 49% H2O, 1% FA). Samples were introduced using a syringe pump (Thermo Fisher Scientific) with a flow rate of 5 μl/min. Xtract algorithm of the Freestyle (Thermo Fischer Scientific, version 1.5) was used to deconvolute the raw data. Deconvolution parameters were set as: output mass, MH+; charge range, 1–5; minimum number detected charge, 2.
8
Reusable 3D-Printed Microfluidic Chip Cleaning
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The 3D-printed cleaning chip was designed to enable the reusability of microfluidic chips by implementing a simple cleaning procedure to reduce biofouling and cross-contamination between the samples. The cleaning procedure utilized a syringe pump and the four channels of the cleaning chip (Figure 2 c,d). First, the biological sample inlet was used: a representative protein sample, 2 mL of bovine serum albumin (BSA) at a high concentration (20 mg/mL), was pumped through the chip, with the cleaning solution and air inlets sealed, using a syringe pump set to 1 mL/min (Thermo Fisher Scientific, Waltham, MA, USA). Following the BSA, air was pumped from the air inlet and through all three non-air channels individually by sealing the remaining two channels, with 0.5 mL of air for each channel, also set at 1 mL/min. The cleaning agent was then pumped, with the biological sample and air inlets sealed, for a total of 10 mL at 1 mL/min. Pure PBS and DI water were tested as the cleaning agent (Thermo Fisher Scientific, Waltham, MA, USA); PBS was determined to be the superior choice. The cleaning procedure ended with a second round of the air to dry all the channels of the chip.
9
Antibacterial Evaluation in G. mellonella
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The protocol used for these assays was inspired by [64 (link)]. Briefly, bacterial strains were cultured in BHI, harvested at OD600 = 0.6, and washed twice in 0.9% NaCl. Cultures were adjusted to reach a density of 2.108, 2.109, 1.108, and 5.106 CFU/mL for MRSA, VRE, CRAB, and CRPA, respectively. Fifteen randomly selected G. mellonella larvae around 280 mg were used for each test group. A 10 µL, an inoculum was injected into the last proleg using a 26 G needle (Terumo, Shibuya City, Japan) and a syringe pump (Thermofisher, Waltham, MA, USA). The inoculum size was checked by plate counting on BHI agar. Saline solution or the 4f compound (based on ¼ MIC or the equivalent of 14 mg/kg for squalamine and 3.5, 14, 1.75, and 7 for 4f in CRAB, CRPA, MRSA, and VRE, respectively) was administered 2 h post-infection. Larvae were incubated at 37 °C and their survival was monitored for 48 h every 2 h, from 16 to 48 h post-infection. Assays were performed in triplicate.
10
Vascular Network Perfusion Validation
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To confirm perfusion in the bonded electrospun membrane with a vascular network, 5wt % 10 µm Fluorescent beads (Thermo Fisher Scientific) in PBS solution was perfused by a syringe pump (New Era Pump System Inc., Farmingdale, NY, USA). The fluorescence of the perfused bead was observed with NIGHTSEA (NIGHTSEA, Lexington, MA, USA).
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