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Anti β tubulin clone 2 28 33

Manufactured by Merck Group
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Anti-β-tubulin (clone 2-28-33) is a monoclonal antibody that specifically recognizes the β-tubulin protein. It is a laboratory research tool used to detect and analyze the distribution and abundance of β-tubulin in various cell and tissue samples.

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Lab products found in correlation

Hoechst, by Thermo Fisher Scientific (2 mentions) Anti ha hrp, by Roche (2 mentions) Anti rpgrip1l, by Proteintech (2 mentions) Anti ift20, by Proteintech (2 mentions) Mouse anti γ tubulin clone gtu 88, by Merck Group (2 mentions) Actub clone 6 11b 1, by Merck Group (2 mentions) Anti rhodopsin clone 4d2, by Merck Group (2 mentions) Anti ehd3, by Abnova (2 mentions) Rabbit anti pericentrin, by Novus Biologicals (2 mentions) Anti ehd2, by Abcam (2 mentions) Anti ehd4, by Abcam (2 mentions) Anti cep290, by Fortis Life Sciences (2 mentions) Anti tmem67, by Proteintech (2 mentions) Goat anti cep164, by Santa Cruz Biotechnology (2 mentions) Anti β actin clone ac 15, by Merck Group (2 mentions) Anti gfp, by Abcam (2 mentions)

2 protocols using anti β tubulin clone 2 28 33

1

Antibody Panel for Cytoskeletal and Ciliary Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercial antibodies used were mouse anti-γ-tubulin (clone GTU-88, 1/5000, Sigma), anti-acetylated α-tubulin (Actub) (clone 6-11B-1, 1/10000, Sigma), anti-rhodopsin (clone 4D2, 1/100), anti-β–actin (clone AC-15, 1/30000, Sigma) and anti-β-tubulin (clone 2-28-33, 1/2000, Sigma), anti-EHD3 (1/1000, Abnova for IF and 1/100, Abcam, 1/500, Proteintech for WB), rabbit anti-pericentrin (1/5000, Novus Biologicals, 1/5000), anti-EHD2 (1/1000, Abcam), anti-EHD4 (1/1000, Abcam), anti-GFP (1/50, Abcam), anti-HA HRP (1:1000, Roche), anti-CEP290 (1/200, Bethyl), anti-RPGRIP1L (1/200, Proteintech), anti-IFT20 (1/200, Proteintech), anti-TMEM67 (1/200, Proteintech), anti-SNAP29 (1/1000 for WB Proteintech and 1/200 for IF from Dr. Andrew Peden) and goat anti-CEP164 (1/500, Santa Cruz). Rabbit anti-Rab11a (1/300) was a gift from Dr. Jim Goldenring and rabbit anti-CP110 (1/500) was a gift from Dr. Monica Diaz. Polyclonal rabbit anti-EHD1 (1/1000) has been previously described33 (link). Fluorescent secondary antibodies and phalloidin (1/50) were from Life Technologies (Molecular probes, Eugene, OR). Nuclei were labeled with Hoechst (Molecular probes, Eugene, OR).
Lu Q., Insinna C., Ott C., Stauffer J., Pintado P.A., Rahajeng J., Baxa U., Walia V., Cuenca A., Hwang Y.S., Daar I.O., Lopes S., Lippincott-Schwartz J., Jackson P.K., Caplan S, & Westlake C.J. (2015). Early steps in primary cilium assembly require EHD1- and EHD3-dependent ciliary vesicle formation. Nature cell biology, 17(3), 228-240.
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2

Antibody Panel for Cytoskeletal and Ciliary Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercial antibodies used were mouse anti-γ-tubulin (clone GTU-88, 1/5000, Sigma), anti-acetylated α-tubulin (Actub) (clone 6-11B-1, 1/10000, Sigma), anti-rhodopsin (clone 4D2, 1/100), anti-β–actin (clone AC-15, 1/30000, Sigma) and anti-β-tubulin (clone 2-28-33, 1/2000, Sigma), anti-EHD3 (1/1000, Abnova for IF and 1/100, Abcam, 1/500, Proteintech for WB), rabbit anti-pericentrin (1/5000, Novus Biologicals, 1/5000), anti-EHD2 (1/1000, Abcam), anti-EHD4 (1/1000, Abcam), anti-GFP (1/50, Abcam), anti-HA HRP (1:1000, Roche), anti-CEP290 (1/200, Bethyl), anti-RPGRIP1L (1/200, Proteintech), anti-IFT20 (1/200, Proteintech), anti-TMEM67 (1/200, Proteintech), anti-SNAP29 (1/1000 for WB Proteintech and 1/200 for IF from Dr. Andrew Peden) and goat anti-CEP164 (1/500, Santa Cruz). Rabbit anti-Rab11a (1/300) was a gift from Dr. Jim Goldenring and rabbit anti-CP110 (1/500) was a gift from Dr. Monica Diaz. Polyclonal rabbit anti-EHD1 (1/1000) has been previously described33 (link). Fluorescent secondary antibodies and phalloidin (1/50) were from Life Technologies (Molecular probes, Eugene, OR). Nuclei were labeled with Hoechst (Molecular probes, Eugene, OR).
Lu Q., Insinna C., Ott C., Stauffer J., Pintado P.A., Rahajeng J., Baxa U., Walia V., Cuenca A., Hwang Y.S., Daar I.O., Lopes S., Lippincott-Schwartz J., Jackson P.K., Caplan S, & Westlake C.J. (2015). Early steps in primary cilium assembly require EHD1- and EHD3-dependent ciliary vesicle formation. Nature cell biology, 17(3), 228-240.
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