Cell apoptosis detection was performed using eBioscience™ Annexin V-FITC/PI Apoptosis Detection Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) with FACS Calibur flow cytometer (BD Biosciences). After 16 h treatment, cells were harvested and washed with the binding buffer from the kit. Then, the cells were resuspended in 100 μL binding buffer containing 3 μL annexin V and incubated for 15 min at RT. After the washing step, 3 μL of propidium iodide was added into 200 μL cell suspension. Cells were analyzed by flow cytometry, and 10,000 events were recorded for each FCM analysis.
Ebioscience annexin 5 fitc pi apoptosis detection kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The EBioscience™ Annexin V-FITC/PI Apoptosis Detection Kit is a laboratory instrument used to detect and quantify apoptosis, a type of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide, a dye that stains DNA, to distinguish between viable, early apoptotic, and late apoptotic/necrotic cells through flow cytometry analysis.
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2 protocols using ebioscience annexin 5 fitc pi apoptosis detection kit
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Annexin V-FITC/PI Apoptosis Assay
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Quantitative Cell Apoptosis Detection
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The cell apoptosis detection was performed using eBioscience™ Annexin V-FITC/PI Apoptosis Detection Kit (Thermo Fisher Scientific, Carlsbad, CA, USA). After 20 h. treatment, cells were harvested and washed with the binding buffer from the kit. Following the manufacturer’s instructions, cells were incubated with Annexin V-FITC and PI sequentially. Finally, all samples were analyzed by NovoCyte Quanteon Flow Cytometer (Agilent Technologies, California, USA), and 10,000 events were recorded for each analysis.
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