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Facscanto 2 facscalibur flow cytometer

Manufactured by BD

The FACSCanto II (FACSCalibur) flow cytometer is a laboratory instrument designed for the analysis and sorting of cells and particles. It utilizes the principles of flow cytometry to rapidly measure and analyze multiple physical and fluorescent characteristics of individual cells or particles as they flow in a fluid stream through a laser beam.

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2 protocols using facscanto 2 facscalibur flow cytometer

1

Exosome Surface Marker Profiling

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Different exosome surface markers were selected based on their functions including: (a) tetraspanins (CD9, CD63 and CD81), (b) targeting/adhesion (CD 31), (c) antigen presentation (HALA-G), and (d) membrane transport and fusion (Rab5a) that were bound to Exo-Flow FACS magnetic beads [(9.1μm), (SBI, System Bioscience, Mountain View, CA)]. Labeled exosomes markers were analyzed on a FACSCanto II (FACSCalibur) flow cytometer (BD Biosciences, San Jose, CA) using the FACSDiva software 2.56 (BD Biosciences).
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2

Exosome Surface Marker Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Different exosome surface markers were selected based on their functions including: (a) tetraspanins (CD9, CD63 and CD81), (b) targeting/adhesion (CD 31), (c) antigen presentation (HALA-G), and (d) membrane transport and fusion (Rab5a) that were bound to Exo-Flow FACS magnetic beads [(9.1μm), (SBI, System Bioscience, Mountain View, CA)]. Labeled exosomes markers were analyzed on a FACSCanto II (FACSCalibur) flow cytometer (BD Biosciences, San Jose, CA) using the FACSDiva software 2.56 (BD Biosciences).
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