Ly6c pe texas red al 2

Peritoneal lavage cells were stained with fluorescently conjugated antibodies to MARCO-fluorescein isothiocyanate (FITC) (MCA1849F; Bio-Rad), CD11c⁻ BV605 (N418), CD86⁻ BV421 (GL-1), CD103⁻ BV510 (M290; BD Biosciences), CD11b⁻ phycoerythrin (PE)-Cy7 (M1/70), Ly6G-PerCP Cy5.5 (1A8), F4/80-APC (BM8), CD45⁻ AF700 (30-F11), MHCII-APC-Cy7 (M5/114.15.2), CD200R⁻ PE (123908), Ly6C-PE-Texas Red (AL-2; BD Biosciences), and NK1.1-BV650 (PK136) and collected on a Fortessa flow cytometer (BD Biosciences). Viability was assessed using 4′,6-diamidino-2-phenylindole (DAPI). Antibodies were purchased from BioLegend unless otherwise stated. Cells were gated based on live cells and small peritoneal macrophages (CD45⁺ F4/80⁺ CD11c⁻ CD11b⁺ MHCII⁺), large peritoneal macrophages (CD45⁺ F4/80⁺ CD11c⁻ CD11b⁺ MHCII⁻), interstitial macrophages (CD45⁺ Ly6C⁻ F4/80⁻ CD11b⁺ CD11c⁺ MHCII⁺), Ly6C-negative monocytes (CD45⁺ Ly6C⁻ F4/80⁻ CD11b⁺ CD11c⁺ MHCII⁻), CD103⁺ dendritic cells (DC) (CD45⁺ Ly6C⁻, F4/80⁻, CD11b⁻, MHCII⁺, CD103⁺ CD11c⁺), C11b⁺ DC (CD45⁺ Ly6C⁺ F4/80⁻ CD11b⁺ MHCII⁺ CD11c⁺), neutrophils (CD45⁺ Ly6C⁺ F4/80⁻ CD11b⁺ MHCII⁻ Ly6G⁺), Ly6C-positive monocytes *CD45⁺ Ly6C⁺ F4/80⁻ Ly6G⁻ CD11b⁺ MHCII⁻), plasmacytoid DC (CD45⁺ Ly6C⁺ F4/80⁻ CD11b⁻ CD11c⁺ MHCII⁺), and natural killer cells (CD45⁺ Ly6G⁻ F4/80⁻ NK1.1⁺).