Log In Sign up
The largest database of trusted experimental protocols

Pro293a medium

Manufactured by Lonza
Visit Supplier

Pro293a medium is a cell culture medium designed for the growth and maintenance of HEK293 cells, a widely used cell line for the production of recombinant proteins and viral vectors. The medium provides the necessary nutrients and supplements to support the proliferation and viability of HEK293 cells in vitro.

Automatically generated - may contain errors

Lab products found in correlation

Pen strep, by Thermo Fisher Scientific (3 mentions) Hyclone dmem, by GE Healthcare (3 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Ni nta agarose, by Qiagen (1 mentions)

Close spelling variants of this product

Pro293a-CDM serum-free medium (2 citations)

4 protocols using pro293a medium

1

Recombinant CEL and CEL-Tn Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant 6xHIS tagged CEL and CEL-Tn (Clone ID: OHu22073C) was expressed in HEK293 and COSMC-KO HEK293-SC (simple cells)3 (link) and purified from Pro293 a Medium (Lonza, Basel, Switzerland) using Ni-NTA agarose (Qiagen).
Wolters-Eisfeld G., Mercanoglu B., Hofmann B.T., Wolpers T., Schnabel C., Harder S., Steffen P., Bachmann K., Steglich B., Schrader J., Gagliani N., Schlüter H., Güngör C., Izbicki J.R., Wagener C, & Bockhorn M. (2018). Loss of complex O-glycosylation impairs exocrine pancreatic function and induces MODY8-like diabetes in mice. Experimental & Molecular Medicine, 50(10), 133.
+ Open protocol
+ Expand
2

HEK293 Cell Efflux Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293 cells were passaged in HyClone DMEM (GE Healthcare Systems, Marlborough, MA) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco, Thermo Fisher, Waltham, MA) at 37°C and 5% CO2 under humidified conditions. Efflux experiments were performed in 6-well plates. 500,000 cells were seeded per well and 2 days later the experiment was started by replacing the culture medium with 2.5 ml Pro293a medium (Lonza, Basel, Switzerland), supplemented with 2 mM L-glutamine and 100 units pen/strep (Gibco, Thermo Fisher, Waltham, MA) per ml. Samples were taken at the indicated time points. At the end of the experiment, the presence of similar numbers of cells was confirmed by sulphorhodamine staining (16 ).
Szeri F., Niaziorimi F., Donnelly S., Fariha N., Tertyshnaia M., Patel D., Lundkvist S, & van de Wetering K. (2022). The mineralization regulator ANKH mediates cellular efflux of ATP, not pyrophosphate. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 37(5), 1024-1031.
+ Open protocol
+ Expand
3

Efflux Assay in HEK293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293 cells were passaged in HyClone DMEM (GE) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco) at 37°C and 5% CO2 under humidified conditions. Efflux experiments were performed in 6-well plates. 500,000 cells were seeded per well and 2 days later the experiment was started by replacing the culture medium with 2.5 ml Pro293a medium (Lonza), supplemented with 2 mM L-glutamine and 100 units pen/strep (Gibco) per ml. Samples were taken at the indicated time points. The presence of equal numbers of cells at the time of the experiment was confirmed by quantifying relative intracellular ATP levels per well (S2 Fig).
Szeri F., Lundkvist S., Donnelly S., Engelke U.F., Rhee K., Williams C.J., Sundberg J.P., Wevers R.A., Tomlinson R.E., Jansen R.S, & van de Wetering K. (2020). The membrane protein ANKH is crucial for bone mechanical performance by mediating cellular export of citrate and ATP. PLoS Genetics, 16(7), e1008884.
+ Open protocol
+ Expand
4

Efflux Experiments in HEK293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293 cells were passaged in HyClone DMEM (GE Healthcare Systems, Marlborough, MA) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco, Thermo Fisher, Waltham, MA) at 37 o C and 5% CO2 under humidified conditions. Efflux experiments were performed in 6-well plates. 500,000 cells were seeded per well and 2 days later the experiment was started by replacing the culture medium with 2.5 ml Pro293a medium (Lonza, Basel, Switzerland), supplemented with 2 mM L-glutamine and 100 units pen/strep (Gibco, Thermo Fisher, Waltham, MA) per ml. Samples were taken at the indicated time points. At the end of the experiment, the presence of similar numbers of cells was confirmed by sulphorhodamine (SRB) staining (20) (link). Correctly targeted clones were identified by Sanger sequencing.
Szeri F., Niaziorimi F., Donnelly S., Fariha N., Tertyshnaia M., Patel D., Lundkvist S., & Wetering K.v.d. (2021). Ankylosis homologue mediates cellular efflux of ATP, not pyrophosphate.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!