F4 80

Manufactured by Dianova

Sourced in Germany

F4/80 is a widely used cell surface marker that is expressed primarily on mature macrophages and tissue-resident macrophages in mice. It is a member of the epidermal growth factor (EGF)-like module-containing mucin-like hormone receptor-like 1 (EMR1) family of G-protein coupled receptors.

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Lab products found in correlation

P stat1, by Cell Signaling Technology (1 mentions) α tubulin, by Merck Group (1 mentions) α actinin, by Merck Group (1 mentions) Sumo2 3, by Cell Signaling Technology (1 mentions) Sumo2 3, by Abcam (1 mentions) Ubiquitin, by Merck Group (1 mentions) P stat3, by Cell Signaling Technology (1 mentions) Histone h3, by Cell Signaling Technology (1 mentions) Stat1, by Cell Signaling Technology (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Gapdh, by Merck Group (1 mentions) Desmin, by Agilent Technologies (1 mentions) D9542, by Merck Group (1 mentions) Alexa conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Immu mount, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) α sma, by Abcam (1 mentions) Sca 1, by Abcam (1 mentions) Vcam 1, by Abcam (1 mentions) Mmp 9, by Abcam (1 mentions)

3 protocols using f4 80

Antibody Dilutions for Western Blot

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Antibodies used in this study are listed below where respective dilutions are mentioned in the parentheses:
α-actinin, mouse monoclonal, Sigma-Aldrich (1:400); SUMO2/3, rabbit monoclonal, Cell signaling (1:1000); SUMO2 + 3, mouse monoclonal, Abcam (1:1000); Calcineurin A, mouse monoclonal, BD Bioscience (1:250); GAPDH, mouse monoclonal, Sigma-Aldrich (1:20,000); Histone H3, rabbit polyclonal, Cell-signaling (1:1000); α-Tubulin, mouse monoclonal, Sigma-Aldrich (1:8000); Ubiquitin, mouse monoclonal, Millipore (1:1000); STAT1, rabbit monoclonal, Cell signaling (1:1000); p-STAT1, rabbit monoclonal, Cell signaling (1:1000); STAT3, rabbit monoclonal, Cell signaling (1:1000); p-STAT3, rabbit monoclonal, Cell signaling (1:1000); F4/80, rat monoclonal, Dianova (1:500); HA, mouse monoclonal, Sigma-Aldrich (1:20,000); HectD3, rabbit polyclonal, Mybiosource (1:1000); V5, mouse monoclonal, Biozol (1:1000).

Rangrez A.Y., Borlepawar A., Schmiedel N., Deshpande A., Remes A., Kumari M., Bernt A., Christen L., Helbig A., Jungmann A., Sossalla S., Tholey A., Müller O.J., Frank D, & Frey N. (2020). The E3 ubiquitin ligase HectD3 attenuates cardiac hypertrophy and inflammation in mice. Communications Biology, 3, 562.

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Histological and Immunohistochemical Analysis

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Paraffin sections (6 mm thick) were stained with hematoxylin and eosin, according to standard procedures for histological analyses. For immunohistochemical stainings, cryosections (6 mm thick) were fixed in either 4% paraformaldehyde or acetone, blocked with 10% normal goat serum or 5% bovine serum albumin (in PBS), and incubated overnight at 4 C with primary antibodies diluted in 10% normal goat serum or 1% bovine serum albumin. Bound primary antibodies were detected by incubation with Alexa-conjugated secondary antibodies for 1 hour at room temperature (Life Technologies, Darmstadt, Germany). Nuclei were stained with DAPI (1:1000, D9542; Sigma-Aldrich, Munich, Germany). Specimens were mounted in Immu-Mount (9990402; Thermo Scientific, Braunschweig, Germany). The following primary antibodies were used: CD31 (1:1000, clone MEC13.3, 557355; BD Biosciences, Heidelberg, Germany); desmin (1:100, clone D33, M0760; Dako, Hamburg, Germany); F4/ 80 (1:200, T-2028; Dianova GmbH, Hamburg, Germany); Ki-67 (1:500, ab15580; Abcam, Cambridge, UK).

Ghatak S., Niland S., Schulz J.N., Wang F., Eble J.A., Leitges M., Mauch C., Krieg T., Zigrino P, & Eckes B. (2016). Role of Integrins α1β1 and α2β1 in Wound and Tumor Angiogenesis in Mice. The American journal of pathology, 186(11).

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Immunohistochemical Analysis of Aortic Markers

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Subsequent to fixation with paraformaldehyde (PFA) 4%, 5-μm-thick aortic sections were incubated with BSA 2.5% and further with antibodies recognizing the markers of interest: α-SMA, MCP-1, MMP2, MMP9, IL-6, VCAM-1, Sca-1, c-Jun (phospho S63) [Y172] (Abcam, Cambridge, UK), cyclin D2 (Biozol, Eching, Germany), ICAM-1 (Santa Cruz, Heidelberg, Germany), F4/80 (Dianova, Hamburg, Germany), and JNK (Invitrogen, Carlsbad, CA, USA). After a series of washing with PBS, incubation with corresponding secondary antibodies (Dianova, Hamburg, Germany) was performed, followed by counterstaining with nuclear DAPI. Fluorescent signals were detected using confocal microscopy (LSM 800, Zeiss, Oberkochen, Germany) and quantified using ImageJ. Four random regions per section and two sections per graft were analyzed.

Remes A., Franz M., Mohr F., Weber A., Rapti K., Jungmann A., Karck M., Hecker M., Kallenbach K., Müller O.J., Arif R, & Wagner A.H. (2019). AAV-Mediated Expression of AP-1-Neutralizing RNA Decoy Oligonucleotides Attenuates Transplant Vasculopathy in Mouse Aortic Allografts. Molecular Therapy. Methods & Clinical Development, 15, 246-256.

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