The extraction of total protein was finished through the usage of RIPA (CWBio, Beijing, China) and the concentrations of proteins were determined through the usage of BCA Protein Quantification Kit (Vazyme). Next, the proteins were loaded on SDS-PAGE gel (Solarbio) and blotted onto PVDF membranes (Amersham Biosciences, Chicago, IL, USA). After blocking in 5% defatted milk for 1 h, the membranes were cultivated overnight with primary antibodies at 4°C followed by incubation with secondary antibody (ab205719; Abcam, Cambridge, MA, USA) for 1 h at indoor temperature. The blots were examined with an ECL detection kit (Pierce Biotechnology, Rockford, IL, USA). The primary antibodies were: Bcl-2 (ab182858; Abcam), Bax (ab32503; Abcam), cleaved-caspase-3 (c-caspase-3; ab49822; Abcam), PRKACB (ab26322; Abcam) and GAPDH (ab9485; Abcam).
Ab26322
Manufactured by Abcam
Ab26322 is a laboratory instrument used for the detection and quantification of specific proteins or other biomolecules in a sample. The core function of this product is to perform various analytical techniques, such as Western blotting or enzyme-linked immunosorbent assay (ELISA), to identify and measure the presence of target molecules.
Automatically generated - may contain errors
Lab products found in correlation
Ab205719, by Abcam (1 mentions)
Ab49822, by Abcam (1 mentions)
Ab182858, by Abcam (1 mentions)
Ab32503, by Abcam (1 mentions)
Radioimmunoprecipitation assay (ripa), by CWBIO (1 mentions)
Pvdf membrane, by Cytiva (1 mentions)
Sds page gel, by Solarbio (1 mentions)
Ecl detection kit, by Thermo Fisher Scientific (1 mentions)
Bca protein quantification kit, by Vazyme (1 mentions)
Ab9485, by Abcam (1 mentions)
Ab52484, by Abcam (1 mentions)
Ab10478, by Abcam (1 mentions)
G6539, by Merck Group (1 mentions)
Formaldehyde, by Electron Microscopy Sciences (1 mentions)
T7451, by Abcam (1 mentions)
T5192, by Santa Cruz Biotechnology (1 mentions)
2 protocols using ab26322
1
Protein Extraction, Quantification, and Western Blot Analysis
2
Immunofluorescence Labeling of Cellular Targets
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Mouse monoclonal antibodies used were anti-GFP ([1:100] Sigma-Aldrich, G6539), acetylated tubulin ([1:1,000] Sigma-Aldrich, T7451) and anti-histone H2B ([1:50] Abcam, ab52484) antibodies. Rabbit polyclonal antibodies used were anti-γ-tubulin ([1:1,000] Sigma-Aldrich, T5192), anti-lamin A/C ([1:20] Santa Cruz Biotechnology, H110), anti-cAMP protein kinase catalytic subunit ([1:1,000] Abcam, ab26322) and anti-CBX/HP1 beta antibodies ([1:100] Abcam, ab10478). TRITC or Alexa Fluor 647 conjugated secondary antibodies (Jackson Immuno Research Laboratories, Inc. or Life Technologies, respectively) were used for indirect immunofluorescence detection. BG-conjugated dyes, including SNAP-Surface Alexa Fluor 647 and 546 (NEB), were used for staining SNAP-tag expressed in cells.
The cells were fixed with 4% formaldehyde (Electron Microscopy Sciences) for 15 min, permeabilized with 0.1% Triton X-100 in phosphate-buffered saline (PBS), stained for primary antibodies and Alexa-Fluor-conjugated secondary antibodies, or BG-conjugated fluorophores for SNAP-tag at 4 °C overnight.
The cells were fixed with 4% formaldehyde (Electron Microscopy Sciences) for 15 min, permeabilized with 0.1% Triton X-100 in phosphate-buffered saline (PBS), stained for primary antibodies and Alexa-Fluor-conjugated secondary antibodies, or BG-conjugated fluorophores for SNAP-tag at 4 °C overnight.
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