Aj100

Manufactured by Mettler Toledo

Sourced in Switzerland

The AJ100 is a precise analytical balance from Mettler Toledo. It is designed for weighing a wide range of samples in laboratory environments. The AJ100 provides accurate and reliable measurements with a readability of 0.1 milligrams.

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Lab products found in correlation

Mz16a, by Leica camera (1 mentions) Eukitt, by Merck Group (1 mentions) Eos 500d, by Canon (1 mentions)

7 protocols using aj100

Harderian Gland Whole Mount Preparation

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Harderian glands from both eyes were isolated and weighed (Mettler-Toledo, AJ100, Greifensee, Switzerland) and their average weight was used for subsequent calculations. For whole mount preparation organs were spread on glass slides and fixed in Carnoy fixative over night at room temperature. After fixation tissue samples were gradually changed to distilled water and stained in carmine and dehydrated in a graded ethanol series, cleared in xylene and mounted in Eukitt (Sigma, 03989). Reagents were prepared as in http://mammary.nih.gov/tools/histological/Histology/ index.html. Photographs were taken on a stereomicroscope (Leica, MZ16 A, Wetzlar, Germany).
Statistical analyses were calculated and blotted in Graphpad Prism in a Student t-test (2 tailed, unpaired samples), the error bars represent SD. N = number of independent biological samples.

Koenig U., Fobker M., Lengauer B., Brandstetter M., Resch G.P., Gröger M., Plenz G., Pammer J., Barresi C., Hartmann C, & Rossiter H. (2014). Autophagy facilitates secretion and protects against degeneration of the Harderian gland. Autophagy, 11(2), 298-313.

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Apical Debris Collection Protocol

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A previously described model by Myers and Montgomery was used for apical debris collection. Eppendorf tubes with their own stoppers were weighed in an electronic weighing machine (Mettler AJ 100, Greifensee, Switzerland) which had an accuracy of 0.0001 g. Three consecutive weights were obtained for each Eppendorf tube, and the average measurement was taken to be its initial weight. Separate rubber stoppers were made for each Eppendorf tube which had a tight fit to its opening, and holes were created on these stoppers such that teeth could be inserted up to the cementoenamel junction, and a 21-G needle was placed alongside the rubber stopper to equalize the internal and external air pressure. These setups were then placed into preweighed Eppendorf tubes, and the tubes were fitted into glass vials [Figure 1].

Ada K.S., Shetty S., Jayalakshmi K.B., Nadig P.L., Manje Gowda P.G, & Selvan A.K. (2022). Influence of different irrigant activation methods on apical debris extrusion and bacterial elimination from infected root canals. Journal of Conservative Dentistry : JCD, 26(1), 31-35.

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Conditioning and Dimensional Analysis

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Each group of samples was placed in a rack inside a desiccator containing dried silica gel. The desiccator was kept in the oven at 37°C for 24 h followed by the transfer of samples to the second desiccator supplied with new silica gel. The second desiccator was kept at 24°C for 1 h then the samples were weighed using an analytical weighing balance (Mettler; AJ‐100). The desiccator remained closed throughout the process except for removing and replacing samples. After weighing all samples, the silica gel in the first desiccator was replaced. The cycle described above was repeated until obtaining a constant mass, called conditioned mass m1, where the loss in mass of each sample is not more than 0.2 mg between two consecutive measurements. After that, the volume (mm³) of each sample was measured by calculating the mean of three diameter measurements and the mean of five thickness measurements at four equally spaced locations at the circumference of the sample, together with a center measurement.

Alanazi K.K., Wood D., Shepherd J., Stokes C.W, & Asencio I.O. (2024). Assessing the suitability of fused deposition modeling to produce acrylic removable denture bases. Clinical and Experimental Dental Research, 10(3), e880.

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Biomass Partitioning in Coastal Plants

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Before the start of the experiment, the initial fresh weight of each Carpobrotus, Artemisia and Helichrysum plant was measured to the nearest 0.0001 g (Mettler AJ100, Mettler-Toledo, Greifensee, Switzerland). The plants were grown for twelve months under the experimental conditions and were then harvested, washed, cleaned and dried at 60 °C to constant weight. Each plant was separated into shoots (including leaves and stolons in the case of Carpobrotus) and roots, and the final dry weights of each fraction (total, above ground and root, dry mass) were recorded.

García C., Campoy J.G, & Retuerto R. (2021). A test of native plant adaptation more than one century after introduction of the invasive Carpobrotus edulis to the NW Iberian Peninsula. BMC Ecology and Evolution, 21, 69.

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Measuring Plant Size and Root Growth

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To determine the size of the plants, rosette area was estimated non-destructively from digital photographs (Canon EOS 500D) of rosettes, taken with a size standard. Image analysis involved converting pixels per rosette into area (mm²), using imaging software (Corel Paintshop Pro, ver. X7). To determine root growth, root material plus soil was collected and oven dried using an economy incubator 2 (Weiss Technik, United Kingdom; 60°C). Subsequently, roots were carefully extracted from the surrounding soil and weighed, using an analytical balance (Mettler Toledo AJ100).

Williams A., Pétriacq P., Beerling D.J., Cotton T.E, & Ton J. (2018). Impacts of Atmospheric CO2 and Soil Nutritional Value on Plant Responses to Rhizosphere Colonization by Soil Bacteria. Frontiers in Plant Science, 9, 1493.

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Measuring Stem Dehydration Dynamics

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The change in weight between rehydrated and dehydrated states of stems was calculated using a Mettler AJ100 analytical balance. Stems were rehydrated overnight and excess water was blotted from the surface of the stem. Individual outer and inner stems were placed upon the balance and allowed to air-dry for 220 and 350 minutes, respectively. The weight loss percentage was calculated with respect to the weight of the stem at its fully hydrated state as w* = 100 × (w_wet − w)/w_wet.

Rafsanjani A., Brulé V., Western T.L, & Pasini D. (2015). Hydro-Responsive Curling of the Resurrection Plant Selaginella lepidophylla. Scientific Reports, 5, 8064.

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Estimating Relative Water Content in Citrus Rootstocks

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As stated above, relative water content (RWC) was estimated at the end of experiment (D21). For this process, two mature and fully expended leaves per plant were selected, with a total of four plants per rootstock and treatment. Then, two discs of 1 cm in diameter per selected leaf were cut and taken. All four discs obtained per each plant were weighed using a precision digital scale (METTLER TOLEDO AJ100, Columbus, OH, USA). Next, each four-disk group was covered with distilled water for 4 h at room temperature and weighed again. After this process, each group of discs was placed in labeled paper enveloped, dried in a heater for 24 h at 80 • C, and finally weighted. The RWC was calculated according to Morgan (1984) (link) [28] (link) with the following equation:
where W = fresh weight of the four discs in each citrus rootstock and treatment, TW = discs turgent weight (after 4 h in distilled water) and DW = dry weight discs.

Aparicio-Durán L., Gmitter F.G., Arjona-López J.M., Grosser J.W., Calero-Velázquez R., Hervalejo Á., & Arenas F.J.A. (2021). Evaluation of Three New Citrus Rootstocks under Boron Toxicity Conditions. Agronomy, 11(12), 2490-2490.

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