Igg cocktail

Manufactured by Roche

The IgG cocktail is a laboratory product designed for the detection and quantification of human immunoglobulin G (IgG) antibodies. It provides a standardized solution of purified human IgG antibodies for use as a reference material in various immunoassay applications.

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Lab products found in correlation

Brij 35, by Thermo Fisher Scientific (2 mentions) Protease inhibitor, by Roche (2 mentions) Slide a lyzer, by Thermo Fisher Scientific (2 mentions) Ez link sulfo nhs lc biotin kit, by Thermo Fisher Scientific (2 mentions)

2 protocols using igg cocktail

Optimized Microarray Assay Buffers

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The buffers and biological solutions used in the microarray assays included the following: 1X phosphate-buffered saline (PBS) + 0.5% or 0.1% Tween-20 (PBST 0.5 or 0.1 ); 10× sample buffer (1× PBS + 1% Tween-20 + 1% Brij-35; Thermo Scientific, Rockford, IL); 4× IgG blocking cocktail (400 μg/mL each of mouse, sheep, and goat IgG, 800 μg/mL rabbit IgG in 1× PBS, antibodies from Jackson Immunoresearch, West Grove, PA); 10× protease inhibitor (Complete Tablet; Roche Applied Science, Indianapolis, IN); and 2× sample dilution buffer (2× sample buffer + 2× protease inhibitor + 2× IgG cocktail in 1× PBS).
The antibodies and lectins were acquired from various sources (Supplementary Table 2). The capture antibodies to be printed onto microarray slides were purified by dialysis (Slide-A-Lyzer; Pierce Biotechnology, Rockford, IL) to 1× PBS and ultracentrifuged. Biotinylation was performed using the EZ-Link-sulfo-NHS-LC-Biotin kit (Pierce Biotechnology) according to the manufacturer’s instructions.

Tang H., Partyka K., Hsueh P., Sinha J.Y., Kletter D., Zeh H., Huang Y., Brand R.E, & Haab B.B. (2015). Glycans Related to the CA19-9 Antigen Are Increased in Distinct Subsets of Pancreatic Cancers and Improve Diagnostic Accuracy Over CA19-9. Cellular and Molecular Gastroenterology and Hepatology, 2(2), 210-221.e15.

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Microarray Assay Buffer Preparation

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The buffers and biological solutions used in the microarray assays included: PBST0.5 or 0.1 (1× phosphate-buffered saline (PBS) + 0.5% or 0.1% Tween-20); 10× sample buffer (1× PBS + 1% Tween-20 + 1% Brij-35 (Thermo Scientific, Rockford, IL)); 4× IgG blocking cocktail (400 μg/ml each of mouse, sheep, and goat IgG, 800 μg/mL rabbit IgG in 1× PBS, antibodies from Jackson Immunoresearch); 10× protease inhibitor (Complete Tablet, Roche Applied Science, Indianapolis, IN); and 2× sample dilution buffer (2× sample buffer + 2× protease inhibitor + 2× IgG cocktail in 1× PBS).
The antibodies and lectins were acquired from various sources (Table S2). The capture antibodies to be printed onto microarray slides were purified by dialysis (Slide-A-Lyzer, Pierce Biotechnology, Rockford, IL) to 1× PBS and ultracentrifuged. Biotinylation was performed using the EZ-Link-sulfo-NHS-LC-Biotin kit (Pierce Biotechnology, Rockford, IL) according to the manufacturer's instructions.

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