For the treatment trial, 8-week-old male littermate BALB/c mice were used. Two 6-mm, circular, full-thickness wounds were generated in each animal by biopsy punch. Donut-shaped 12-mm silicone splints (Grace Bio-Labs, Bend, OR, USA) were placed around the wounds and affixed with glue and sutures to prevent wound contraction.12 Plastic collars were used to prevent the mice from tearing off the splints.12 The mice that underwent surgery were divided into three groups. The first group consisted of mice treated with CA IV; purified CA IV was mixed with 30% Pluronic-127 (Sigma-Aldrich) gel as previously described,13 (link) and the pH was 7.0. Fifty microliters of the mixture was added to each wound to fill up the entire wound cavity. The second group received a CA inhibitor, acetazolamide (1.0 mM , Orion, Espoo, Finland), in Pluronic-127 gel. The third group served as a control group and was treated with 50 μl of phosphate-buffered saline mixed with Pluronic-127 gel. The final concentration of Pluronic-127 in each solution was 18%. Each mouse was photographed after the silicone splints had been securely sutured in place and then killed on day 5.
Pluronic 127
Manufactured by Merck Group
Sourced in United States, Germany
Pluronic 127 is a non-ionic surfactant that is commonly used in various laboratory applications. It is a triblock copolymer consisting of polyethylene oxide (PEO) and polypropylene oxide (PPO) segments. Pluronic 127 is known for its ability to form micelles and act as a solubilizing agent, emulsifier, and dispersant in aqueous solutions.
Automatically generated - may contain errors
Lab products found in correlation
Donut shaped 12 mm silicone splints, by Grace Bio-Labs (1 mentions)
Stearic acid, by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Agarose, by GeneDireX (1 mentions)
Dopamine, by Merck Group (1 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (1 mentions)
Copper 2 chloride, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Acetone, by Avantor (1 mentions)
Trichloromethane, by Merck Group (1 mentions)
Octanol, by Merck Group (1 mentions)
1 2 dichloroethane, by Merck Group (1 mentions)
N decane, by Merck Group (1 mentions)
Mgcl2, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
N hexane, by Merck Group (1 mentions)
Cacl2, by Merck Group (1 mentions)
Mgso4, by Merck Group (1 mentions)
7 protocols using pluronic 127
1
Carbonic Anhydrase IV Promotes Wound Healing
2
Chitosan-Based Nanoparticle Formulation
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DFL was purchased from Beijing Mesochem Technology (Beijing, China). Stearic acid, chitosan and pluronic 127 were purchased from Sigma Aldrich (St. Louis, MO, USA). All chemicals and solvents used in this study were analytical/HPLC grade. Ultra-pure water was collected from the Milli-Q water purifier unit (Millipore, Darmstadt, Germany) and was used for aqueous solution preparation.
3
Copper-Catalyzed Oxidation Assay Protocol
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Copper(II) chloride (CuCl2), hydrogen peroxide (H2O2, ~ 30%), Tris base, Pluronic® 127, dopamine, 3,3’,5,5’-tetramethylbenzidine (TMB), and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ammonium hydroxide (NH4OH), ethanol (~ 99%), acetone (~ 99%), and mesitylene were purchased from J.T. Baker® (Pennsylvania, USA) and Alfa Aesar (Heysham, Lancashire) respectively. Cell culture-related products, including Dulbecco’s Modified Eagle Medium (DMEM, CAT: CC103-0500), RPMI 1640 medium (CAT: CC110-0500), and agarose, were obtained from GeneDireX, Inc. Penicillin-streptomycin (10,000 U/mL, CAT: 154140-122) and fetal bovine serum (FBS, CAT: 10437-028) were procured from Gibco®. Interleukin-2 (IL-2, CAT: 50,792-M08H) was procured from Sino Biological.
4
Synthesis of Alumina Nanopowders
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Alumina powders (diameter: 200–300
nm) were purchased from Baikowski. Dolapix was purchased from Zschimmer
& Schwarz. Pluronic 127, octanol, 1,2-dichloroethane, trichloromethane,
n-decane, n-hexane, sapphire, and other reagents, including NaCl,
CaCl2, MgCl2, MgSO4, were of analytical
reagent grade and obtained from Sigma-Aldrich. All reagents were used
directly and did not require further purification.
nm) were purchased from Baikowski. Dolapix was purchased from Zschimmer
& Schwarz. Pluronic 127, octanol, 1,2-dichloroethane, trichloromethane,
n-decane, n-hexane, sapphire, and other reagents, including NaCl,
CaCl2, MgCl2, MgSO4, were of analytical
reagent grade and obtained from Sigma-Aldrich. All reagents were used
directly and did not require further purification.
5
Synthesis of Fluorescent Lipid Nanoparticles
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CIP, potassium hydroxide (KOH), acetic anhydride, DMSO, DCM, sodium cyanoborohydride (NaBH4), oils, surfactants, co-surfactants, pluronic 127, culture media, fluorescent dyes, and Schiff reagent were obtained from Sigma-Aldrich, Germany. All solvents used were of analytical grade.
6
Drosophila S2U cell culture and RNAi treatment
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Drosophila S2U cells were maintained in Schneider’s
medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum
(Sigma-Aldrich), penicillin, streptomycin, and kanamycin (Goshima et al., 2007 (link)). Full-length DE-cadherin was cloned
into pAc5.1/V5–His B (Invitrogen) and cotransfected with blasticidin and
hygromycin plasmids in S2U cells to generate stable expression lines (Millar et al., 1994 (link)). For RNAi treatment,
DE-cadherin–expressing S2 cells were treated with 625 µg/ml
trypsin for 20 min at 25°C and then resuspended in serum-free
Schneider’s medium. 1 µg RNA was incubated with 6 ×
105 cells for 30 min in serum-free Schneider’s medium
followed by a 5-d recovery in Schneider’s medium supplemented with 20%
heat-inactivated fetal bovine serum (Goshima
et al., 2007 ). RNAi-treated cells were maintained in plasticware
coated with a 10% Pluronic 127 (Sigma-Aldrich) solution to minimize
cell–substrate interactions. MDCK G type II cells were grown in DMEM with
1 g/liter sodium bicarbonate, 10% fetal bovine serum (Atlas Biologicals),
penicillin, streptomycin, and kanamycin. For MDCK cells, two rounds of 10
µg siRNA was transfected (Lipofectamine 2000) for 18-h periods, and cells
were analyzed after a 24-h recovery.
medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum
(Sigma-Aldrich), penicillin, streptomycin, and kanamycin (Goshima et al., 2007 (link)). Full-length DE-cadherin was cloned
into pAc5.1/V5–His B (Invitrogen) and cotransfected with blasticidin and
hygromycin plasmids in S2U cells to generate stable expression lines (Millar et al., 1994 (link)). For RNAi treatment,
DE-cadherin–expressing S2 cells were treated with 625 µg/ml
trypsin for 20 min at 25°C and then resuspended in serum-free
Schneider’s medium. 1 µg RNA was incubated with 6 ×
105 cells for 30 min in serum-free Schneider’s medium
followed by a 5-d recovery in Schneider’s medium supplemented with 20%
heat-inactivated fetal bovine serum (
et al., 2007
coated with a 10% Pluronic 127 (Sigma-Aldrich) solution to minimize
cell–substrate interactions. MDCK G type II cells were grown in DMEM with
1 g/liter sodium bicarbonate, 10% fetal bovine serum (Atlas Biologicals),
penicillin, streptomycin, and kanamycin. For MDCK cells, two rounds of 10
µg siRNA was transfected (Lipofectamine 2000) for 18-h periods, and cells
were analyzed after a 24-h recovery.
7
Curcumin and Pluronic 127 Nanoformulation
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Malic acid, 1, 12-Dodecanedioic acid (DDA), Pluronic 127 were purchased from Sigma. Curcumin and Glycerol were purchased from TCI and Himedia, respectively. Primary (caspase-9 and GAPDH) and secondary antibodies were procured from Santa Cruz, US. Other chemicals used in animal cell culture studies, such as Dulbecco’s modified Eagle’s medium (DMEM), Fetal bovine serum (FBS), Trypsin-EDTA were purchased from Gibco, Thermo Fisher Scientific, India. Phosphate buffer saline, Acridine orange, Ethidium bromide and DAPI were obtained from Himedia, India. MCF-7 and MDA-MB-231 cell lines were procured from NCCS, Pune, India.
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