Imagequant tl iqtl 8

Southern blotting was performed according to our previously reported methods [15 (link)]. Briefly, Hirt DNA samples were resolved on 1% agarose gel, and the separated DNAs were transferred onto a supported nitrocellulose membrane (#1212590, GVS North America, ME) and probed with an [α-³²P] dCTP-labeled probe of the HBoV1 NSCap gene [24 (link)]. HBoV1 DNA excised from SalI and XhoI-digested pIHBoV1 plasmid was used as the probe template. Hybridization signals were captured by using a storage phosphor screen and visualized on an Amersham Typhoon Biomolecular Imager (Cytiva). Signals were quantified using ImageQuant Tl (IQTL) 8.2 (Cytiva).

Southern blotting was essentially performed according to our previously reported methods (85 (link)). Briefly, lower molecular weight (Hirt) DNA samples were prepared from cells as described (75 (link)), and resolved in 1% agarose gel. The separated DNAs were transferred onto a nitrocellulose membrane (#1212590; GVS North America) and probed with an [α-³²P]-dCTP-labeled probe of the AAV2 repcap gene. In some experiments, the blot was additionally probed for mitochondrial DNA (mtDNA) using a specific probe (32 (link)). After overnight exposure with a storage phosphor screen, hybridization signals were captured and visualized on an Amersham Typhoon Biomolecular Imager (Cytiva), and quantified using ImageQuant Tl (IQTL) 8.2 (Cytiva).