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Mk2206

Manufactured by MedChemExpress
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Sourced in United States, China, Germany

MK2206 is a laboratory compound used for research purposes. It functions as an inhibitor of the AKT kinase, a key regulator of cellular processes such as metabolism, proliferation, and survival. The core function of MK2206 is to modulate AKT activity in experimental settings. No additional details or interpretations about the intended use of this product are provided.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (10 mentions) Ly294002, by MedChemExpress (6 mentions) Rapamycin, by MedChemExpress (5 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) Pan akt, by Cell Signaling Technology (3 mentions) U0126, by MedChemExpress (3 mentions) Rad001, by MedChemExpress (3 mentions) Rpmi 1640, by Thermo Fisher Scientific (3 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (3 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Gdc 0941, by MedChemExpress (2 mentions) Vinculin, by Merck Group (2 mentions) Her2 and her3, by Santa Cruz Biotechnology (2 mentions) Anti phosphotyrosine antibody clone 4g10 agarose conjugate, by Merck Group (2 mentions) Immunofluorescently labeled antibody to mouse igg, by Rockland Immunochemicals (2 mentions) Antibody to rabbit igg, by Thermo Fisher Scientific (2 mentions) P mek, by Cell Signaling Technology (2 mentions) Odyssey scanner, by LI COR (2 mentions) Ripa buffer, by Merck Group (2 mentions)

75 protocols using mk2206

1

Inhibition of Akt and Smad3 Signaling

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To inhibit Akt signaling, cells were seeded in 24-well plates and cultured for 15 min, 30 min, 60 min, 24 h, 3 d, and 7 d in low-serum medium with water and dimethyl sulfoxide (DMSO) (vehicle controls), and low-serum medium supplemented with 50 ng/mL recombinant human TGFβ2 (PeproTech), 500 nM of the Akt inhibitor MK-2206 [36 (link)] (MedChem Express, Monmouth Junction, NJ), or both (TGFβ2 + MK-2206). To inhibit Smad3 signaling, cells were cultured and seeded into 24-well plates as described above for 15 min, 30 min, 60 min, 24 h, 3 d, and 7 d in low-serum medium with water and DMSO (vehicle controls), and low-serum medium supplemented with 50 ng/mL recombinant human TGFβ2 (PeproTech), 2 μM of the Smad3 inhibitor SIS3 [11 (link)] (Tocris, Bristol, UK), or both (TGFβ2 + SIS3).
Theodossiou S.K., Murray J.B., Hold L.A., Courtright J.M., Carper A.M, & Schiele N.R. (2021). Akt signaling is activated by TGFβ2 and impacts tenogenic induction of mesenchymal stem cells. Stem Cell Research & Therapy, 12, 88.
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2

Combination Therapy for SMAD4-Deficient Colorectal Cancer

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The Institutional Animal Care and Use Committee at Boston University School of Medicine approved all animal experiments. Six-week old female athymic nude mice (Nu/Nu) were purchased from Envigo and housed in a sterile environment with microisolator cages. The mice were subcutaneously injected with 2.5 × 106 HCT116 SMAD4−/− cells in 30% growth factor-reduced Matrigel (Corning). When the tumors reached around 5mm in diameter, the mice were exposed to vehicle, MK2206 (MedChemExpress) alone, irinotecan (MedChemExpress) alone, or a combination of MK2206 and irinotecan. MK2206 (360mg/kg) in 30% Captisol was administered on days 1, 8, 15, and 22 via oral gavage. irinotecan (20mg/kg) was administered on days 1, 8, 15, and 22 via intraperitoneal injections. Tumor volume was determined using (L x W2)/2, where L represents length and W represents width.
Wong C.K., Lambert A.W., Ozturk S., Papageorgis P., Lopez D., Shen N., Sen Z., Abdolmaleky H.M., Győrffy B., Feng H, & Thiagalingam S. (2020). Targeting RICTOR sensitizes SMAD4-negative colon cancer to irinotecan. Molecular cancer research : MCR, 18(3), 414-423.
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3

Establishing Venetoclax-Resistant AML Cell Lines

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Human AML cell lines, U937 and MV4–11 (CRL-9591) were purchased from the ATCC (Manassas, Virginia, USA), MOLM-13 (DSMZ Cat# ACC-554) and OCI-AML3 (DSMZ Cat# ACC-582) cells were purchased from DSMZ (Brunswick, Lower Saxony, Germany) and maintained as described previously [25 (link)]. All cell lines were tested for Mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza) routinely. All experiments utilized logarithmically growing cells (3–4×105 cells/ml).
Venetoclax-resistant MV4–11 and MOLM-13 cells were obtained by culturing in the presence of increasing venetoclax (from 1nM to 1μM) concentrations over a period of 2 months.
Venetoclax was a gift from AbbVie (Chicago, IL, USA). INK128, AZD2014 was purchased from ChemieTek (Indianapolis, IN, USA). copanlisib was purchased from AdooQ (Irvine, CA, USA). MK2206 was purchased from MedChemExpress (Monmouth Junction, NJ, USA). Rapamycin was purchased from Cell Signaling (Danvers, MA, USA). All drugs were dissolved in DMSO, aliquoted, and stored at −80. Final DMSO concentrations did not exceed 0.1%.
Satta T., Li L., Chalasani S.L., Hu X., Nkwocha J., Sharma K., Kmieciak M., Rahmani M., Zhou L, & Grant S. (2023). Dual mTORC1/2 inhibition synergistically enhances AML cell death in combination with the BCL2 antagonist venetoclax. Clinical cancer research : an official journal of the American Association for Cancer Research, 29(7), 1332-1343.
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4

Dextrose and Astragaloside IV Effects on T84 Cells

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T84 cells were obtained from the Culture Collection of the Chinese Academy of Sciences, Shanghai, China. T84 cells were cultured in DMEM/F-12 (BIOAGRIO, Shanghai, China) medium supplemented with 10% fetal bovine serum (Gibco, Gaithersburg, MD, USA) at 37 °C with 5% humidified CO2. After 3-5 passages, T84 cells were seeded into 6-well plates, and experiments were performed when a 50–60% confluence was reached. The cells were exposed to 4.25% dextrose-containing PDF and AS IV (1, 5, and 10 μM) for various time periods. Subsequently, MK-2206 (AKT inhibitor, MedChemExpress, Princeton, NJ, USA) dissolved in DMSO at a concentration of 5 μM was added to the AS IV (5 μM) adminstration group to identify the role of AKT in this setting.
He J., Wang M., Yang L., Xin H., Bian F., Jiang G, & Zhang X. (2022). Astragaloside IV Alleviates Intestinal Barrier Dysfunction via the AKT-GSK3β-β-Catenin Pathway in Peritoneal Dialysis. Frontiers in Pharmacology, 13, 873150.
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5

NCAPG and CKII Regulation in Proliferation

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The NCAPG overexpression plasmid (pcDNA3.1( +)-NCAPG) and corresponding negative control vector (pcDNA3.1( +)-NC), NCAPG knockdown plasmid (shNCAPG) and control (shNC), and CKII overexpression (pcDNA3.1( +)-CKII) and knockdown plasmids (shCKII) were ordered from GeneChem (Shanghai, China). MK2206 and TBB were purchased from MedChemExpress (Monmouth Junction, NJ, USA). In general, the final concentrations of MK2206 and TBB were 25 µM and 10 µM respectively. The cells were treated with drugs for 24 h before proliferation-related tests or western blot analysis. The transfection reagent used for transient transfer was Lipofectamine 3000 (Invitrogen, Thermo Fisher Scientific, Inc., USA). For stable transfer, GeneChem (Shanghai, China) was used.
Zhang R., Ai J., Wang J., sun C., Lu H., He A., Li M., Liao Y., Lei J., Zhou F., Wu L, & Liao W. (2022). NCAPG promotes the proliferation of hepatocellular carcinoma through the CKII-dependent regulation of PTEN. Journal of Translational Medicine, 20, 325.
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6

Oral MK2206 administration before hypercapnia and IAV

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MK2206 (MedChemExpress LLC) was administrated by oral gavage at 120 mg/kg body weight one day before hypercapnia exposure and then one day before IAV infection. Captisol (MedChemExpress LLC) was used as a vehicle for the drug and control animals were treated with vehicle only.
Casalino-Matsuda S.M., Chen F., Gonzalez-Gonzalez F.J., Nair A., Dib S., Yemelyanov A., Gates K.L., Budinger G.R., Beitel G.J, & Sporn P.H. (2020). Hypercapnia Suppresses Macrophage Antiviral Activity and Increases Mortality of Influenza A Infection via Akt1. Journal of immunology (Baltimore, Md. : 1950), 205(2), 489-501.
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7

Cytokine and Inhibitor Evaluation in Cancer Cells

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Recombinant Human TNF-α (Cat. No. 300-01A), IFN-γ (Cat. No. 300-02), and insulin growth factor (IGF; Cat. No. 100-11) were purchased from PeproTech (Cranbury, NJ, USA). Sodium butyrate (Cat. No. S1200) and cisplatin (Cat. No. C2210000) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). The inhibitors CUCD-101 (Cat. No. HY-10223), decitabine (HY-A0004), santacruzmate A (HY-N0931), zebularine (HY-13420), olaparib (HY-10162), alpelisib (HY-15244), and MK-2206 (HY-108232) were all purchased from MedChemExpress (Sollentuna, Sweden). Fulvestrant (ICI 182,780; Cat. No. 1047) was obtained from Tocris Bioscience (Bristol, UK). A medium containing 0.5% DMSO was used as a vehicle-only control for the experiments.
Wörthmüller J., Disler S., Pradervand S., Richard F., Haerri L., Ruiz Buendía G.A., Fournier N., Desmedt C, & Rüegg C. (2023). MAGI1 Prevents Senescence and Promotes the DNA Damage Response in ER+ Breast Cancer. Cells, 12(15), 1929.
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8

Akt Pathway Activation and Inhibition Assays

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IGF-1, BCA Kit, penicillin/streptomycin mix, and Puromycin were purchased from Beijing Soleibao Company (Beijing, China). Akt specific inhibitor (MK-2206) was from MedChem Express (Monmouth, NJ, USA). Adenovirus encoding the human Akt S473D mutant carboxyterminally tagged 3 copies of flag epitope (1.2 × 1010 pfu/mL) and the GFP adenovirus (1.5 × 1010 pfu/mL) were from WZ Biosciences Inc. (Jinan, Shandong, China). Lipofectamine 3000 was from Thermo Fisher Scientific (Waltham, MA, USA). G418 was from Gibco (Grand Island, New York, NY, USA). Transwell chamber and matrigel were from BD Biosciences (San Jose, CA, USA). Protein A/G-agarose was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). CCK8 was from AbMole (Houston, TX, USA). Myc-agarose was Sigma-Aldrich (St. Louis, MI, USA). HyClone Dulbecco’s Modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Cytiva (Marlborough, MA, USA). All antibodies with catalog number and manufactures are listed in Table 1.
Chen Q., Jin J., Guo W., Tang Z., Luo Y., Ying Y., Lin H, & Luo Z. (2022). PEBP4 Directs the Malignant Behavior of Hepatocellular Carcinoma Cells via Regulating mTORC1 and mTORC2. International Journal of Molecular Sciences, 23(15), 8798.
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9

Modulation of NDRG1 and GSK3B Signaling

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NDRG1 or GSK3B genes were inhibited by transient transfection with siRNA (siNDRG1 and siGSK3β) from Santa Cruz (50 ng/ml) and lipofectamine RNAiMAX (Invitrogen) following the manufacturer's instructions for 48h after stimulation with TGFβ1 (10 ng/ml) (PeproTech) for 8h or 14 days. TGFβ1 was replenished every 72h. Scrambled siRNA (SCR) was used as negative control 20 (link). Galunisertib (LY2157299, TGFβR1 inhibitor) (5 μM), CHIR99021 (GSK3α/β inhibitor) (10 μM), LY294002 (PI3K inhibitor) (10 μM), MK2206 (Akt inhibitor) (1 μM), GSK650394 (SGK1/2 inhibitor) (10 μM), and Rapamycin (mTOR inhibitor) (10 μM) were from MedChemExpress. Cells were pre-stimulated with TGFβ1 for 8h and treated with each inhibitor for 24, 48, and 72h.
López-Tejada A., Griñán-Lisón C., González-González A., Cara F.E., Luque R.J., Rosa-Garrido C., Blaya-Cánovas J.L., Navarro-Ocón A., Valenzuela-Torres M., Parra-López M., Calahorra J., Blancas I., Marchal J.A, & Granados-Principal S. (2023). TGFβ Governs the Pleiotropic Activity of NDRG1 in Triple-Negative Breast Cancer Progression. International Journal of Biological Sciences, 19(1), 204-224.
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10

Naïve T Cell Activation and Treg Differentiation

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Mice were sacrificed, their spleens were removed and gently dissociated into single-cell suspensions. Naïve T cells were isolated using the MojoSort Mouse CD4 Naïve T Cell Isolation Kit (BioLegend). T cells were cultured in U-bottom 96-well plates with RPMI 1640 culture medium supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco), 2 mM l-glutamine, 1% antibiotic-antimycotic (Gibco), 10 mM HEPES (Gibco), 1× nonessential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), and 55 μM β-mercaptoethanol (Gibco). Naïve T cells were activated with plate-bound anti-CD3 (3 μg/ml; Bio X Cell) and soluble anti-CD28 (2 μg/ml; Bio X Cell) antibodies. Treg cell differentiation was achieved by the indicated concentration of recombinant human TGF-β (R&D). The Akt inhibitor MK-2206 and afuresertib were purchased from MedChemExpress (MCE). Cells were cultured for 72 h and detected by flow cytometry.
Cai W., Zhang J., Zhou H., Li X., Lou F., Sun Y., Xu Z., Bai J., Yin Q., Wang Z., Sun L., Cai X., Tang S., Wu Y., Fan L., Wang H., Wang H, & Li Q. (2021). Protein phosphatase 6 (Pp6) is crucial for regulatory T cell function and stability in autoimmunity. Genes & Diseases, 9(2), 562-575.
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