Triglycerides lq

Lipase, amylase and aspartate aminotransferase (AST) activity were determined in plasma by the LIPASE-LQ, AMYLASE-LQ and GOT (AST)-LQ, respectively, (Spinreact, Girona, Spain). The procedures were performed according to the indications of the kit.
Triglycerides and total lipids were determined in liver tissue by the TRIGLYCERIDES-LQ and TOTAL LIPIDS, respectively (Spinreact, Girona, Spain). The procedures were performed according to the indications of the kit in liver homogenate in PBS (100 mg/mL). Results were normalized by protein concentration.

After 25 weeks, rats from both groups were weighed, fasted 12 h, and sacrificed. Blood samples were collected using K+EDTA as anticoagulant. Retroperitoneal adipose tissue was collected and weighed. Plasma was obtained by blood centrifugation (3000 rpm during 15 minutes at 4°C) and stored at –70°C until needed. Glucose was measured with a commercial enzymatic kit (DCL-glucose oxidase Diagnostic Chemical Limited de Mexico, Mexico). Insulin was determined with a commercial rat specific radioimmunoassay kit (Linco Research, Inc., Missouri, USA) with 0.1 ng/mL sensitivity and intra- and interassay coefficients of variation of 5 and 10%, respectively. Triglycerides and cholesterol were determined with commercially available procedures (Spinreact cholesterol-LQ and triglycerides-LQ; Spinreact S.A. Girona, Spain). HDL-cholesterol was measured by enzymatic procedures (Hitachi 902 analyzer; Hitachi LTD, Tokyo, Japan). Accuracy and precision of lipid measurements in our laboratory are under periodic surveillance as recommended by the Centers for Disease Control and Prevention (Atlanta, GA, USA). Plasma endotoxin levels were determined with GenScript Toxin Chromogenic LAL Endotoxin according to the manufacturer's instructions.

Quantification of glucose (Glucose-LQ, Spinreact, Girona, Spain), triglycerides (Triglycerides-LQ, Spinreact, Girona, Spain), and uric acid (Sekisui Diagnostics, Burlington, MA, USA) was performed using commercial kits according to the manufacturer’s instructions.