Hepg2 cells

Manufactured by GE Healthcare

Sourced in United States, Germany

HepG2 cells are a well-established human liver cancer cell line commonly used in laboratory research. They are derived from a hepatocellular carcinoma and exhibit characteristics of normal human hepatocytes. HepG2 cells are a valuable tool for studying liver biology, metabolism, and the effects of drugs and other compounds on liver function.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by GE Healthcare (1 mentions) Dulbecco modified eagle medium (dmem), by GE Healthcare (1 mentions) Penicillin streptomycin, by GE Healthcare (1 mentions) Rpmi 1640 medium, by GE Healthcare (1 mentions) Tryple select, by Thermo Fisher Scientific (1 mentions) Amphotericin b, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Clark Bioscience (1 mentions) Dmem high glucose, by GE Healthcare (1 mentions) Bovine serum albumin (bsa), by Solarbio (1 mentions) Amphotericin, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)

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HepG2 (5 citations)

5 protocols using hepg2 cells

Adropin Regulation in Hepatoma Cells

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The human hepatoma cell line (HepG2 cells) obtained from the Culture Collection and Research Center of the Food Industry Institute (BCRC No. 60,025) were cultured in the Dulbecco’s modified Eagle’s medium (DMEM) (GE Healthcare Life Sciences, Pittsburgh, PA, USA) containing 10% fetal bovine serum (GE Healthcare Life Sciences, Pittsburgh, PA, USA), 1% penicillin/streptomycin (GE Healthcare Life Sciences) under standard conditions (95% air, 5% CO₂, 37 °C). HepG2 cells (1×10⁶ cells/dish) were seeded in the 10-cm culture dishes.
To investigate the change of adropin in HepG2 cells under high-glucose conditions, cells were incubated in serum-free medium with varying concentrations of D-glucose (10, 20 or 30 mM) for 48 h. HepG2 cells exposed to normal medium containing 5.5 mM glucose were used as the controls for comparison. HepG2 cells treated with the culture medium supplemented with 30 mM mannitol to get an osmotic control as 30 mM glucose.

Kuo F.Y., Cheng K.C., Li Y., Cheng J.T, & Tsai C.C. (2020). Promotion of Adropin Expression by Hyperglycemia Is Associated with STAT3 Activation in Diabetic Rats. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 13, 2269-2277.

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HepG2 Cell Culture Conditions

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Human hepatoblastoma HepG2 cells (DMSZ, Braunschweig, Germany) were cultured in RPMI 1640 medium (GE Healthcare, PAA Laboratories, Pasching, Austria) supplemented with 10% fetal bovine serum (FBS Superior, Biochrome, Berlin, Germany) and 50 units/ml penicillin and 50 μg/ml streptomycin (Gibco by lifetechnologies, Carlsbad, CA, USA) at 37°C in a 5% CO₂ atmosphere.

Haenisch S., Zhao Y., Chhibber A., Kaiboriboon K., Do L.V., Vogelgesang S., Barbaro N.M., Alldredge B.K., Lowenstein D.H., Cascorbi I, & Kroetz D.L. (2015). SOX11 identified by target gene evaluation of miRNAs differentially expressed in focal and non-focal brain tissue of therapy-resistant epilepsy patients. Neurobiology of disease, 77, 127-140.

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HepG2 Cell Culture Protocol

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HepG2 cells (ATCC) were cultured in Minimum Essential Medium Alpha Modification (αMEM; GE Healthcare) supplemented with 10% (v/v) fetal bovine serum (Gibco), 100 IU/mL penicillin and 0.1 mg/mL streptomycin (Gibco) and 1.25 μg/ml amphotericin B (Gibco). Cell media was replaced every 48 h, and at approximately 80% confluence cells were harvested or passaged with TrypLE^TM Select (Gibco).

Mendonça da Silva J., Erro E., Awan M., Chalmers S.A., Fuller B, & Selden C. (2020). Small-Scale Fluidized Bed Bioreactor for Long-Term Dynamic Culture of 3D Cell Constructs and in vitro Testing. Frontiers in Bioengineering and Biotechnology, 8, 895.

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Liver Cancer Cell Line HepG2 Cultivation and Treatment

Cited 1 time

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Liver cancer HepG2 cells, purchased from the American Type Culture Collection, were cultured in high-glucose DMEM (GE Healthcare Life Sciences) with 10% fetal bovine serum (Clark Bioscience) and 1% penicillin/streptomycin at 37°C in an atmosphere containing 5% CO₂. The cells were dissociated with 0.25% trypsin (w/v) and 0.52 mM EDTA [M&C Gene Technology (Bejing) Ltd.] and routinely sub-cultured at 80% confluency.
Cell cultures were treated with various concentrations of apigenin in DMSO as the carrier solvent. Palmitic acid binds to fatty-acid-free BSA (Beijing Solarbio Science & Technology Co., Ltd.). In brief, palmitic acid was dissolved in 1X PBS and a 250 mM stock solution was obtained following various cycles of incubation in a water bath at 70°C and vortexing. The stock solution was then added to serum-free DMEM containing 5% fatty-acid-free BSA to obtain a 250 µM palmitic acid solution, and the resulting diluted solution was used for the cell treatments (16 (link),17 (link)). AMPK inhibitor compound C was diluted with DMSO to a final concentration of 10 µM and was used to verify the signaling pathway.

Lu J., Meng Z., Cheng B., Liu M., Tao S, & Guan S. (2019). Apigenin reduces the excessive accumulation of lipids induced by palmitic acid via the AMPK signaling pathway in HepG2 cells. Experimental and Therapeutic Medicine, 18(4), 2965-2971.

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HepG2 Cells 3D Encapsulation in FFP

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HepG2 cells (ECACC Wiltshire) were cultured in modified α-MEM (GE Healthcare) supplemented with 10% Fetal Bovine Serum (FBS) (GIbco), 1% Penicillin/Streptomycin (Gibco) and 0.5% Amphotericin (Gibco), to grow sufficient cells in monolayer to seed subsequent 3D culture. Media was changed every 2-3 days.
After encapsulation the AELS were cultured in media containing Fresh Frozen Plasma (FFP) in place of FBS.

Awan M., Erro E., Forster-Brown E., Brookshaw T., Chandel S., Chalmers S.A., Watt A., Fuller B, & Selden C. (2022). Dimethyl sulfoxide for cryopreservation of alginate encapsulated liver cell spheroids in bioartificial liver support; assessments of cryoprotectant toxicity tolerance and dilution strategies. Cryobiology, 106.

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