Lpg 3400sd pump

Determination of water-soluble active components in TDC was carried out using a Thermo U-3000 series HPLC system equipped with an Lpg-3400sd pump, WPS-3000 automatic sampler, TCC-3100 column, and photodiode array detector (or diode array detector). Chromatographic separation was achieved on an Elit reversed-phase column C 18 column (4.6 × 150 mm, 5 μm) maintained at 30°C. Methanol (solvent A) and 0.1% phosphoric acid in water (solvent B) served as one of the mobile phases. The gradient program is as follows: 0–10 min, 5–10% A; 10–20 min, 10–20% A; 20–30 min, 20–30% A; 30–40 min, 30–40% A; 40–50 min, 40–50% A; 50–55 min, 55–5% A; 55–65 min, remain 5% A. The detection wavelength was set at 245 nm. Acetonitrile (solvent A) and deionized water (solvent B) served as another mobile phase. The gradient program was as follows: 0–22 min, 19% A; 22–60 min, 19–36% A; 60–65 min, 36–19% A; 65–75 min, remain19% A. The detection wavelength was set at 203 nm. A flow rate of 1.0 ml/min was utilized. An aliquot of 20 μl sample solution was injected for analysis.

The morphological characterization of Mag-CCNT-TEPA was carried out by scanning electron microscope (SEM, Zeiss Sigma 300, Germany), and the chemical composition was carried out by X-ray photoelectron spectrometer (XPS, Thermo Scientific K-Alpha, USA). The magnetic characteristics of the material were determined by a vibrating sample magnetometer (VSM, LakeShore 7404, USA). The crystal structure characterization was conducted by X-ray diffraction (XRD, Panalytical X’Pert’3 Powder, Germany).
The separation and analysis of seven local anesthetic drugs were performed on an Ultimate 3000 HPLC system equipped with an LPG-3400SD pump, six-port valve with a 20 μL sample loop, TCC-100 thermostat column compartment, and VWD-3400 variable-wavelength UV detector (Thermo Scientific, USA). The deionized water used in all experiments was purified by a water-purification apparatus (Thermo Scientific, USA).

A slight reduction in the lipid concentration may occur during dispersion production by dilution with process water remaining in the homogenization device. In order to achieve the lipid mixing ratio of 9 CN + 1 TM accurately, the lipid content of the unloaded dispersions was determined by HPLC after preparation. A Dionex UltiMate 3000 HPLC system (Thermo Fisher Scientific, Waltham, MA, USA) equipped with an LPG-3400SD pump, a WPS-3000TSL autosampler, and a Corona Veo Charged Aerosol detector was used to perform the analysis. The column (Thermo Fisher Scientific Hypersil Gold C18, 2.1 × 150 mm, 1.9 μm) was kept at 25 °C and the flow rate was set to 0.3 mL/min. The mobile phase consisted of acetonitrile/tetrahydrofuran 70/30 (v/v). Under these conditions, the retention time of both lipids was between 3 and 5 min, with cholesteryl nonanoate eluting from the column prior to trimyristin.
For sample preparation, dispersions were dissolved in tetrahydrofuran/acetonitrile 50/50 (v/v) and diluted to an appropriate detector response; 1 μL was injected and detected at a nebulizer temperature of 50 °C. Every sample was diluted twice and every dilution measured two times (n = 4). Lipid concentrations were calculated with the Chromeleon 7.2 software (Thermo Fisher Scientific, Waltham, MA, USA) using a calibration curve for trimyristin or cholesteryl nonanoate in different concentrations.